One-step separation of CD20+ cells from whole blood using bacterial magnetic particles displaying protein G

Masayuki Takahashi, Tomoko Yoshino, Haruko Takeyama, Tadashi Matsunaga

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Magnetic separation of target cells from mixtures, such as peripheral blood and bone marrow, has considerable practical potential in research and medical applications. Among the current cell separation techniques, magnetic cell separation using immunomagnetic particles has been routinely applied and has proven rapidness and simplicity. Magnetospirillum magneiicum AMB-1 synthesizes intracellular nano-sized bacterial magnetic particles (BacMPs) that are individually enveloped by a stable lipid bilayer membrane. BacMPs, which exhibit strong ferrimagnetism, can be collected easily with commercially available permanent magnets. In this study, a novel magnetic nanoparticle displaying protein G (protein G-BacMPs) was fabricated, and one-step cell separation for direct cell separation from whole blood was performed using the protein G-BacMPs. B lymphocytes (CD20+ cells), which cover less than 0.3 × 10-2 % of whole blood cells, were separated with 93% purity using protein G-BacMPs binding with anti-CD20 monoclonal antibodies. The results of this study demonstrate the utility of protein G-BacMPs and the magnetic cell separation approach based on protein G-BacMPs in numerous applications.

Original languageEnglish
Title of host publicationMaterials Research Society Symposium Proceedings
Pages98-103
Number of pages6
Volume1094
Publication statusPublished - 2008
Externally publishedYes
Event2008 MRS Spring Meeting - San Francisco, CA
Duration: 2008 Mar 242008 Mar 28

Other

Other2008 MRS Spring Meeting
CitySan Francisco, CA
Period08/3/2408/3/28

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ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering
  • Mechanics of Materials

Cite this

Takahashi, M., Yoshino, T., Takeyama, H., & Matsunaga, T. (2008). One-step separation of CD20+ cells from whole blood using bacterial magnetic particles displaying protein G. In Materials Research Society Symposium Proceedings (Vol. 1094, pp. 98-103)