One-trial conditioned taste aversion in Lymnaea: Good and poor performers in long-term memory acquisition

Rio Sugai, Sachiyo Azami, Hatsuki Shiga, Takayuki Watanabe, Hisayo Sadamoto, Suguru Kobayashi, Dai Hatakeyama, Yutaka Fujito, Ken Lukowiak, Etsuro Ito

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

In the majority of studies designed to elucidate the causal mechanisms of memory formation, certain members of the experimental cohort, even though subjected to exactly the same conditioning procedures, remember significantly better than others, whereas others show little or no long-term memory (LTM) formation. To begin to address the question of why this phenomenon occurs and thereby help clarify the causal mechanism of LTM formation, we used a conditioned taste aversion (CTA) procedure on individuals of the pond snail Lymnaea stagnalis and analyzed their subsequent behavior. Using sucrose as an appetitive stimulus and KCl as an aversive stimulus, we obtained a constant ratio of 'poor' to 'good' performers for CTA-LTM. We found that approximately 40% of trained snails possessed LTM following a one-trial conditioning procedure. When we examined the time-window necessary for the memory consolidation, we found that if we cooled snails to 4°C for 30 min within 10 min after the one-trial conditioning, LTM was blocked. However, with delayed cooling (i.e. longer than 10 min), LTM was present. We could further interfere with LTM formation by inducing inhibitory learning (i.e. backward conditioning) after the one-trial conditioning. Finally, we examined whether we could motivate snails to acquire LTM by depriving them of food for 5 days before the one-trial conditioning. Food-deprived snails, however, failed to exhibit LTM following the one-trial conditioning. These results will help us begin to clarify why some individuals are better at learning and forming memory for specific tasks at the neuronal level.

Original languageEnglish
Pages (from-to)1225-1237
Number of pages13
JournalJournal of Experimental Biology
Volume210
Issue number7
DOIs
Publication statusPublished - 2007 Apr
Externally publishedYes

Fingerprint

Lymnaea
Long-Term Memory
conditioned behavior
Snails
conditioning
snail
snails
Learning
trial
Food
learning
Conditioning (Psychology)
Lymnaea stagnalis
Sucrose
food
sucrose
consolidation

Keywords

  • Cooling
  • Long-term memory
  • Motivation
  • One-trial conditioning

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

One-trial conditioned taste aversion in Lymnaea : Good and poor performers in long-term memory acquisition. / Sugai, Rio; Azami, Sachiyo; Shiga, Hatsuki; Watanabe, Takayuki; Sadamoto, Hisayo; Kobayashi, Suguru; Hatakeyama, Dai; Fujito, Yutaka; Lukowiak, Ken; Ito, Etsuro.

In: Journal of Experimental Biology, Vol. 210, No. 7, 04.2007, p. 1225-1237.

Research output: Contribution to journalArticle

Sugai, R, Azami, S, Shiga, H, Watanabe, T, Sadamoto, H, Kobayashi, S, Hatakeyama, D, Fujito, Y, Lukowiak, K & Ito, E 2007, 'One-trial conditioned taste aversion in Lymnaea: Good and poor performers in long-term memory acquisition', Journal of Experimental Biology, vol. 210, no. 7, pp. 1225-1237. https://doi.org/10.1242/jeb.02735
Sugai, Rio ; Azami, Sachiyo ; Shiga, Hatsuki ; Watanabe, Takayuki ; Sadamoto, Hisayo ; Kobayashi, Suguru ; Hatakeyama, Dai ; Fujito, Yutaka ; Lukowiak, Ken ; Ito, Etsuro. / One-trial conditioned taste aversion in Lymnaea : Good and poor performers in long-term memory acquisition. In: Journal of Experimental Biology. 2007 ; Vol. 210, No. 7. pp. 1225-1237.
@article{48164186714541e28e3ca46a7fd77b26,
title = "One-trial conditioned taste aversion in Lymnaea: Good and poor performers in long-term memory acquisition",
abstract = "In the majority of studies designed to elucidate the causal mechanisms of memory formation, certain members of the experimental cohort, even though subjected to exactly the same conditioning procedures, remember significantly better than others, whereas others show little or no long-term memory (LTM) formation. To begin to address the question of why this phenomenon occurs and thereby help clarify the causal mechanism of LTM formation, we used a conditioned taste aversion (CTA) procedure on individuals of the pond snail Lymnaea stagnalis and analyzed their subsequent behavior. Using sucrose as an appetitive stimulus and KCl as an aversive stimulus, we obtained a constant ratio of 'poor' to 'good' performers for CTA-LTM. We found that approximately 40{\%} of trained snails possessed LTM following a one-trial conditioning procedure. When we examined the time-window necessary for the memory consolidation, we found that if we cooled snails to 4°C for 30 min within 10 min after the one-trial conditioning, LTM was blocked. However, with delayed cooling (i.e. longer than 10 min), LTM was present. We could further interfere with LTM formation by inducing inhibitory learning (i.e. backward conditioning) after the one-trial conditioning. Finally, we examined whether we could motivate snails to acquire LTM by depriving them of food for 5 days before the one-trial conditioning. Food-deprived snails, however, failed to exhibit LTM following the one-trial conditioning. These results will help us begin to clarify why some individuals are better at learning and forming memory for specific tasks at the neuronal level.",
keywords = "Cooling, Long-term memory, Motivation, One-trial conditioning",
author = "Rio Sugai and Sachiyo Azami and Hatsuki Shiga and Takayuki Watanabe and Hisayo Sadamoto and Suguru Kobayashi and Dai Hatakeyama and Yutaka Fujito and Ken Lukowiak and Etsuro Ito",
year = "2007",
month = "4",
doi = "10.1242/jeb.02735",
language = "English",
volume = "210",
pages = "1225--1237",
journal = "Journal of Experimental Biology",
issn = "0022-0949",
publisher = "Company of Biologists Ltd",
number = "7",

}

TY - JOUR

T1 - One-trial conditioned taste aversion in Lymnaea

T2 - Good and poor performers in long-term memory acquisition

AU - Sugai, Rio

AU - Azami, Sachiyo

AU - Shiga, Hatsuki

AU - Watanabe, Takayuki

AU - Sadamoto, Hisayo

AU - Kobayashi, Suguru

AU - Hatakeyama, Dai

AU - Fujito, Yutaka

AU - Lukowiak, Ken

AU - Ito, Etsuro

PY - 2007/4

Y1 - 2007/4

N2 - In the majority of studies designed to elucidate the causal mechanisms of memory formation, certain members of the experimental cohort, even though subjected to exactly the same conditioning procedures, remember significantly better than others, whereas others show little or no long-term memory (LTM) formation. To begin to address the question of why this phenomenon occurs and thereby help clarify the causal mechanism of LTM formation, we used a conditioned taste aversion (CTA) procedure on individuals of the pond snail Lymnaea stagnalis and analyzed their subsequent behavior. Using sucrose as an appetitive stimulus and KCl as an aversive stimulus, we obtained a constant ratio of 'poor' to 'good' performers for CTA-LTM. We found that approximately 40% of trained snails possessed LTM following a one-trial conditioning procedure. When we examined the time-window necessary for the memory consolidation, we found that if we cooled snails to 4°C for 30 min within 10 min after the one-trial conditioning, LTM was blocked. However, with delayed cooling (i.e. longer than 10 min), LTM was present. We could further interfere with LTM formation by inducing inhibitory learning (i.e. backward conditioning) after the one-trial conditioning. Finally, we examined whether we could motivate snails to acquire LTM by depriving them of food for 5 days before the one-trial conditioning. Food-deprived snails, however, failed to exhibit LTM following the one-trial conditioning. These results will help us begin to clarify why some individuals are better at learning and forming memory for specific tasks at the neuronal level.

AB - In the majority of studies designed to elucidate the causal mechanisms of memory formation, certain members of the experimental cohort, even though subjected to exactly the same conditioning procedures, remember significantly better than others, whereas others show little or no long-term memory (LTM) formation. To begin to address the question of why this phenomenon occurs and thereby help clarify the causal mechanism of LTM formation, we used a conditioned taste aversion (CTA) procedure on individuals of the pond snail Lymnaea stagnalis and analyzed their subsequent behavior. Using sucrose as an appetitive stimulus and KCl as an aversive stimulus, we obtained a constant ratio of 'poor' to 'good' performers for CTA-LTM. We found that approximately 40% of trained snails possessed LTM following a one-trial conditioning procedure. When we examined the time-window necessary for the memory consolidation, we found that if we cooled snails to 4°C for 30 min within 10 min after the one-trial conditioning, LTM was blocked. However, with delayed cooling (i.e. longer than 10 min), LTM was present. We could further interfere with LTM formation by inducing inhibitory learning (i.e. backward conditioning) after the one-trial conditioning. Finally, we examined whether we could motivate snails to acquire LTM by depriving them of food for 5 days before the one-trial conditioning. Food-deprived snails, however, failed to exhibit LTM following the one-trial conditioning. These results will help us begin to clarify why some individuals are better at learning and forming memory for specific tasks at the neuronal level.

KW - Cooling

KW - Long-term memory

KW - Motivation

KW - One-trial conditioning

UR - http://www.scopus.com/inward/record.url?scp=34248366418&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34248366418&partnerID=8YFLogxK

U2 - 10.1242/jeb.02735

DO - 10.1242/jeb.02735

M3 - Article

C2 - 17371921

AN - SCOPUS:34248366418

VL - 210

SP - 1225

EP - 1237

JO - Journal of Experimental Biology

JF - Journal of Experimental Biology

SN - 0022-0949

IS - 7

ER -