One-trial conditioning of aerial respiratory behaviour in Lymnaea stagnalis

Kara Martens, Martin Amarell, Kashif Parvez, Karla Hittel, Pascaline De Caigny, Etsuro Ito, Ken Lukowiak

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Repeated spaced training sessions of contingent tactile stimulation to the pneumostome as it opens are required to cause long-term memory (LTM) formation of aerial respiratory behaviour making if difficult to determine exactly when memory forms. We have devised a single-trial aversive operant conditioning training procedure in Lymnaea to be better able to elucidate the causal mechanisms of LTM formation. Observations of baseline breathing behaviour in hypoxia were first made. Twenty-four hours later the snails were trained using the single trial procedure, by placing them in a small Petri dish containing 4 ml of 25 mM KCl for 30-35 s as soon as the first pneumostome opening in hypoxia was attempted. LTM was present if (1) breathing behaviour following training was significantly less than before; and (2) breathing behaviour post-training was significantly less in experimental groups than in yoked control groups. LTM persisted for 24 h but not 48 h. Yoked controls that received an aversive stimulus not contingent with pneumostome opening had no evidence of memory. Cooling directly after, but not at any other time, blocks LTM formation. LTM formation was also prevented by removal of the cell body of the neuron RPeD1 before training.

Original languageEnglish
Pages (from-to)232-242
Number of pages11
JournalNeurobiology of Learning and Memory
Volume88
Issue number2
DOIs
Publication statusPublished - 2007 Sep
Externally publishedYes

Fingerprint

Lymnaea
Long-Term Memory
Respiration
Operant Conditioning
Snails
Touch
Conditioning (Psychology)
Neurons
Control Groups

Keywords

  • Invertebrate
  • Learning
  • Long-term memory
  • LTM
  • Lymnaea stagnalis
  • Mollusc
  • One-trial learning
  • Operant training
  • Single-trial training
  • Stress

ASJC Scopus subject areas

  • Behavioral Neuroscience
  • Cognitive Neuroscience
  • Experimental and Cognitive Psychology

Cite this

Martens, K., Amarell, M., Parvez, K., Hittel, K., De Caigny, P., Ito, E., & Lukowiak, K. (2007). One-trial conditioning of aerial respiratory behaviour in Lymnaea stagnalis. Neurobiology of Learning and Memory, 88(2), 232-242. https://doi.org/10.1016/j.nlm.2007.04.009

One-trial conditioning of aerial respiratory behaviour in Lymnaea stagnalis. / Martens, Kara; Amarell, Martin; Parvez, Kashif; Hittel, Karla; De Caigny, Pascaline; Ito, Etsuro; Lukowiak, Ken.

In: Neurobiology of Learning and Memory, Vol. 88, No. 2, 09.2007, p. 232-242.

Research output: Contribution to journalArticle

Martens, K, Amarell, M, Parvez, K, Hittel, K, De Caigny, P, Ito, E & Lukowiak, K 2007, 'One-trial conditioning of aerial respiratory behaviour in Lymnaea stagnalis', Neurobiology of Learning and Memory, vol. 88, no. 2, pp. 232-242. https://doi.org/10.1016/j.nlm.2007.04.009
Martens, Kara ; Amarell, Martin ; Parvez, Kashif ; Hittel, Karla ; De Caigny, Pascaline ; Ito, Etsuro ; Lukowiak, Ken. / One-trial conditioning of aerial respiratory behaviour in Lymnaea stagnalis. In: Neurobiology of Learning and Memory. 2007 ; Vol. 88, No. 2. pp. 232-242.
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