Oocyte-specific linker histone H1foo is an epigenomic modulator that decondenses chromatin and impairs pluripotency

Koji Hayakawa, Jun Ohgane, Satoshi Tanaka, Shintaro Yagi, Kunio Shiota

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Mammalian oocytes contain the histone h1foo, a distinct member with low sequence similarity to other members in the h1 histone family. Oocyte-specific h1foo exists until the second embryonic cell stage. h1foo is essential for oocyte maturation in mice; however, the molecular function of this h1 subtype is unclear. To explore the function of h1foo, we generated embryonic stem (ES) cells ectopically expressing h1foo fused to an EGFp (h1foo-ES). Interestingly, ectopic expression of h1foo prevented normal differentiation into embryoid bodies (EBs). The EB preparations from h1foo-ES cells maintained the expression of pluripotent marker genes, including Nanog, Myc and Klf9, and prevented the shift of the DNa methylation profile. Because the short hairpin RNa-mediated knockdown of h1foo-EGFp recovered the differentiation ability, h1foo was involved in preventing differentiation. Furthermore, chIp analysis revealed that h1foo- EGFp bound selectively to a set of hypomethylated genomic loci in h1foo-ES, clearly indicating that these loci were targets of h1foo. Finally, nuclease sensitivity assay suggested that h1foo made these target loci decondensed. We concluded that h1foo has an impact on the genome-wide, locus-specific epigenetic status.

Original languageEnglish
Pages (from-to)1029-1036
Number of pages8
JournalEpigenetics
Volume7
Issue number9
DOIs
Publication statusPublished - 2012 Sep
Externally publishedYes

Fingerprint

Epigenomics
Histones
Chromatin
Oocytes
Embryoid Bodies
Embryonic Stem Cells
Methylation
Genome
Genes

Keywords

  • Differentiation
  • DNA methylation
  • Histone H1
  • Nuclease-sensitivity
  • Oocyte-specific

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research

Cite this

Oocyte-specific linker histone H1foo is an epigenomic modulator that decondenses chromatin and impairs pluripotency. / Hayakawa, Koji; Ohgane, Jun; Tanaka, Satoshi; Yagi, Shintaro; Shiota, Kunio.

In: Epigenetics, Vol. 7, No. 9, 09.2012, p. 1029-1036.

Research output: Contribution to journalArticle

Hayakawa, Koji ; Ohgane, Jun ; Tanaka, Satoshi ; Yagi, Shintaro ; Shiota, Kunio. / Oocyte-specific linker histone H1foo is an epigenomic modulator that decondenses chromatin and impairs pluripotency. In: Epigenetics. 2012 ; Vol. 7, No. 9. pp. 1029-1036.
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