Optic nerve stimulation-induced increase of release of 3h-glutamate and 3h-aspartate but not 3h-gaba from the suprachiasmatic nucleus in slices of rat hypothalamus

Shyh Yuh Liou, Shigenobu Shibata, Katsunori Iwasaki, Showa Ueki

Research output: Contribution to journalArticle

153 Citations (Scopus)

Abstract

This study investigates the possibility of glutamate and/or aspartate as an excitatory transmitter in the retinohypothalamic fibers' terminal within the anterior hypothalamus, including the suprachiasmatic nucleus (SCN). We studied the release of [3]H-Glu and 3H-Asp from the SCN of rat hypothalamic slices produced by optic nerve stimulation. Stimulation of the optic nerve released 3H-Glu, 3H-Asp but not 3H-GABA, while stimulation of the SCN released all three. These releases were suppressed in both Ca2+ free Krebs and tetrodotoxin (TTX) 1 μM containing Krebs. The present result with previous electrophysiological observations strongly suggest that glutamate and/or aspartate are included in this retinohypothalamic termination, while GABA is included in intrinsic and/or extrinsic neurons of the SCN, excluding the terminal of the retinohypothalamic fibers.

Original languageEnglish
Pages (from-to)527-531
Number of pages5
JournalBrain Research Bulletin
Volume16
Issue number4
DOIs
Publication statusPublished - 1986
Externally publishedYes

Fingerprint

Suprachiasmatic Nucleus
Optic Nerve
Aspartic Acid
Hypothalamus
Glutamic Acid
gamma-Aminobutyric Acid
Anterior Hypothalamus
Tetrodotoxin
Neurons

Keywords

  • Electrophysiology
  • Excitatory amino acids
  • Release experiment
  • Retinohypothalamic pathway
  • Suprachiasmatic nucleus

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Optic nerve stimulation-induced increase of release of 3h-glutamate and 3h-aspartate but not 3h-gaba from the suprachiasmatic nucleus in slices of rat hypothalamus. / Liou, Shyh Yuh; Shibata, Shigenobu; Iwasaki, Katsunori; Ueki, Showa.

In: Brain Research Bulletin, Vol. 16, No. 4, 1986, p. 527-531.

Research output: Contribution to journalArticle

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AU - Iwasaki, Katsunori

AU - Ueki, Showa

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AB - This study investigates the possibility of glutamate and/or aspartate as an excitatory transmitter in the retinohypothalamic fibers' terminal within the anterior hypothalamus, including the suprachiasmatic nucleus (SCN). We studied the release of [3]H-Glu and 3H-Asp from the SCN of rat hypothalamic slices produced by optic nerve stimulation. Stimulation of the optic nerve released 3H-Glu, 3H-Asp but not 3H-GABA, while stimulation of the SCN released all three. These releases were suppressed in both Ca2+ free Krebs and tetrodotoxin (TTX) 1 μM containing Krebs. The present result with previous electrophysiological observations strongly suggest that glutamate and/or aspartate are included in this retinohypothalamic termination, while GABA is included in intrinsic and/or extrinsic neurons of the SCN, excluding the terminal of the retinohypothalamic fibers.

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