Optical manipulation of the alpha subunits of heterotrimeric G proteins using photoswitchable dimerization systems

Gaigai Yu, Hiroyuki Onodera, Yuki Aono, Fuun Kawano, Yoshibumi Ueda, Akihiro Furuya, Hideyuki Suzuki, Moritoshi Sato

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Alpha subunits of heterotrimeric G proteins (Gα) are involved in a variety of cellular functions. Here we report an optogenetic strategy to spatially and temporally manipulate Gα in living cells. More specifically, we applied the blue light-induced dimerization system, known as the Magnet system, and an alternative red light-induced dimerization system consisting of Arabidopsis thaliana phytochrome B (PhyB) and phytochrome-interacting factor 6 (PIF6) to optically control the activation of two different classes of Gα (Gαq and Gαs). By utilizing this strategy, we demonstrate successful regulation of Ca2+ and cAMP using light in mammalian cells. The present strategy is generally applicable to different kinds of Gα and could contribute to expanding possibilities of spatiotemporal regulation of Gα in mammalian cells.

Original languageEnglish
Article number35777
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 2016 Oct 21
Externally publishedYes

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Heterotrimeric GTP-Binding Proteins
Dimerization
Light
Phytochrome B
Optogenetics
Phytochrome
Magnets
Arabidopsis

ASJC Scopus subject areas

  • General

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Optical manipulation of the alpha subunits of heterotrimeric G proteins using photoswitchable dimerization systems. / Yu, Gaigai; Onodera, Hiroyuki; Aono, Yuki; Kawano, Fuun; Ueda, Yoshibumi; Furuya, Akihiro; Suzuki, Hideyuki; Sato, Moritoshi.

In: Scientific Reports, Vol. 6, 35777, 21.10.2016.

Research output: Contribution to journalArticle

Yu, Gaigai ; Onodera, Hiroyuki ; Aono, Yuki ; Kawano, Fuun ; Ueda, Yoshibumi ; Furuya, Akihiro ; Suzuki, Hideyuki ; Sato, Moritoshi. / Optical manipulation of the alpha subunits of heterotrimeric G proteins using photoswitchable dimerization systems. In: Scientific Reports. 2016 ; Vol. 6.
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