Optimum dialysis membrane for endotoxin blocking

Masayo Hayama, Takehiro Miyasaka, Seiichi Mochizuki, Hiroko Asahara, Ken Ichiro Yamamoto, Fukashi Kohori, Katsuhiko Tsujioka, Kiyotaka Sakai

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    We have reported a novel method of visualizing endotoxin (Et) distribution inside an Et-blocking filtration membrane using both fluorescence-labeled Et and a confocal laser scanning fluorescence microscope (CLSFM) in our previous paper [J. Membr. Sci. 210 (2002) 45]. The objective of the present study is to clarify Et-blocking mechanism of dialysis membranes. Six kinds of dialysis membranes with varying materials (hydrophilic and hydrophobic) and varying structures (pore diameter, skin layer location and thickness, and water content) were evaluated by CLSFM together with other techniques such as atomic force microscopy (AFM). Physicochemical property of a membrane material affects Et-adsorbing efficiency, and further membrane structure affects Et-plugging efficiency. Rejected Et distribution in the membranes with varying materials and structures is successfully visualized using fluorescence-labeled Et by CLSFM. Et adsorption on the membranes occurs first, followed by the narrowing of their pores, and afterward pore plugging is continued. Adsorption plays a vital role in Et-blocking. Double skin layer structure is valid for preventing of Et contamination than only inner skin layer structure because the double skin layer structure blocks Et more farther from blood-side surfaces than the only inner skin layer structure.

    Original languageEnglish
    Pages (from-to)15-25
    Number of pages11
    JournalJournal of Membrane Science
    Volume219
    Issue number1-2
    DOIs
    Publication statusPublished - 2003 Jul 15

    Fingerprint

    endotoxins
    Dialysis membranes
    dialysis
    Endotoxins
    Dialysis
    Skin
    Fluorescence
    membranes
    Membranes
    Microscopes
    Scanning
    Lasers
    fluorescence
    Adsorption
    Membrane structures
    plugging
    Pore structure
    Water content
    microscopes
    Atomic force microscopy

    Keywords

    • Atomic force microscopy
    • Confocal laser scanning fluorescence microscopy
    • Endotoxin
    • Hemodialysis
    • Highly permeable dialysis membranes

    ASJC Scopus subject areas

    • Filtration and Separation
    • Polymers and Plastics

    Cite this

    Hayama, M., Miyasaka, T., Mochizuki, S., Asahara, H., Yamamoto, K. I., Kohori, F., ... Sakai, K. (2003). Optimum dialysis membrane for endotoxin blocking. Journal of Membrane Science, 219(1-2), 15-25. https://doi.org/10.1016/S0376-7388(03)00131-5

    Optimum dialysis membrane for endotoxin blocking. / Hayama, Masayo; Miyasaka, Takehiro; Mochizuki, Seiichi; Asahara, Hiroko; Yamamoto, Ken Ichiro; Kohori, Fukashi; Tsujioka, Katsuhiko; Sakai, Kiyotaka.

    In: Journal of Membrane Science, Vol. 219, No. 1-2, 15.07.2003, p. 15-25.

    Research output: Contribution to journalArticle

    Hayama, M, Miyasaka, T, Mochizuki, S, Asahara, H, Yamamoto, KI, Kohori, F, Tsujioka, K & Sakai, K 2003, 'Optimum dialysis membrane for endotoxin blocking', Journal of Membrane Science, vol. 219, no. 1-2, pp. 15-25. https://doi.org/10.1016/S0376-7388(03)00131-5
    Hayama M, Miyasaka T, Mochizuki S, Asahara H, Yamamoto KI, Kohori F et al. Optimum dialysis membrane for endotoxin blocking. Journal of Membrane Science. 2003 Jul 15;219(1-2):15-25. https://doi.org/10.1016/S0376-7388(03)00131-5
    Hayama, Masayo ; Miyasaka, Takehiro ; Mochizuki, Seiichi ; Asahara, Hiroko ; Yamamoto, Ken Ichiro ; Kohori, Fukashi ; Tsujioka, Katsuhiko ; Sakai, Kiyotaka. / Optimum dialysis membrane for endotoxin blocking. In: Journal of Membrane Science. 2003 ; Vol. 219, No. 1-2. pp. 15-25.
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