Optimum drift velocity for single molecule fluorescence bursts in micro/nano-fluidic channels

Lazar L. Kish*, Jun Kameoka, Claes G. Granqvist, Laszlo B. Kish

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Photonic burst histograms can be used to identify single protein molecules in micro/nano-fluidic channels provided the width of the histogram is narrow. Photonic shot noise and residence time fluctuations, caused by longitudinal diffusion, are the major sources of the histogram width. This paper is a sequel to an earlier one of ours [L. L. Kish, Appl. Phys. Lett. 99, 143121 (2011)] and demonstrates that, for a given diffusion coefficient, an increase of the drift velocity enhances the relative shot noise and decreases the relative residence time fluctuations. This leads to an optimum drift velocity that minimizes the histogram width and maximizes the ability to identify single molecules, which is an important result for applications.

Original languageEnglish
Article number043120
JournalApplied Physics Letters
Volume101
Issue number4
DOIs
Publication statusPublished - 2012 Jul 23
Externally publishedYes

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

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