Optofluidic single molecule flow proteometry

Nan Jing*, Chao Kai Chou, Mien Chie Hung, Jun Kameoka

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A microfluidic single molecule fluorescence-based detection scheme is developed to identify target proteindirect from cell lysate by using polyclonal antibody. Relative concentration of target protein in solution is determined by twodimensional(2D) photon burst analysis. Compared to conventional ensemble measurement assays, this microfluidic single molecule approach combines the advantages of higher sensitivity, fast processing time, small sample consumption and high resolution quantitative analysis.

Original languageEnglish
Title of host publicationSingle Molecule Spectroscopy and Imaging II
DOIs
Publication statusPublished - 2009
Externally publishedYes
EventSingle Molecule Spectroscopy and Imaging II - San Jose, CA, United States
Duration: 2009 Jan 242009 Jan 25

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7185
ISSN (Print)1605-7422

Conference

ConferenceSingle Molecule Spectroscopy and Imaging II
Country/TerritoryUnited States
CitySan Jose, CA
Period09/1/2409/1/25

Keywords

  • Flow proteometry
  • Fluorescence
  • Microfluidics
  • Single molecule detection

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Fingerprint

Dive into the research topics of 'Optofluidic single molecule flow proteometry'. Together they form a unique fingerprint.

Cite this