Origin of magnetosome membrane: Proteomic analysis of magnetosome membrane and comparison with cytoplasmic membrane

Masayoshi Tanaka, Yoshiko Okamura, Atsushi Arakaki, Tsuyoshi Tanaka, Haruko Takeyama, Tadashi Matsunaga

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

Prokaryotes are known to have evolved one or more unique organelles. Although several hypotheses have been proposed concerning the biogenesis of these intracellular components, the majority of these proposals remains unclear. Magnetotactic bacteria synthesize intracellular magnetosomes that are enclosed by lipid bilayer membranes. From the identification and characterization of several surface and transmembrane magnetosome proteins, we have postulated that magnetosomes are derived from the cytoplasmic membrane (CM). To confirm this hypothesis, a comparative proteomic analysis of the magnetosome membrane (MM) and CM of the magnetotactic bacterium, Magnetospirillum magneticum AMB-1, was undertaken. Based on the whole genome sequence of M. magneticum AMB-1, 78 identified MM proteins were also found to be prevalent in the CM, several of which are related to magnetosome biosynthesis, such as Mms13, which is tightly bound on the magnetite surface. Fatty acid analysis was also conducted, and showed a striking similarity between the CM and MM profiles. These results suggest that the MM is derived from the CM.

Original languageEnglish
Pages (from-to)5234-5247
Number of pages14
JournalProteomics
Volume6
Issue number19
DOIs
Publication statusPublished - 2006 Oct 1
Externally publishedYes

Keywords

  • Magnetosome
  • Magnetospirillum magneticum AMB-1
  • Magnetotactic bacteria
  • Proteome analysis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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