Abstract
Automated DNA sequencers draw the four-base profiles of a sample with four different colors, but it is also possible to draw the profiles of a base-specific reaction of four different samples with four colors. PCR-amplified DNAs from four individuals were subjected to a single base-specific sequencing reaction and the products were applied to a set of four lanes of an automated DNA sequencer. A base substitution in an individual was clearly identified as an individual-specific peak with a color specific for the individual. In this way, we analyzed more than 50 individuals and identified several polymorphic base substitutions. The sensitivity of this method was high enough to allow detection of the mutation/polymorphism even if samples from several individuals were applied to one lane. Thus, our method is applicable to screening of a large number of samples in an automated manner.
| Original language | English |
|---|---|
| Pages (from-to) | 415-417 |
| Number of pages | 3 |
| Journal | Genomics |
| Volume | 15 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1993 Feb |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
Cite this
Orphan Peak Analysis : A Novel Method for Detection of Point Mutations Using an Automated Fluorescence DNA Sequencer. / Hattori, Masahira; Shibata, Asami; Yoshioka, Katsuji; Sakaki, Yoshiyuki.
In: Genomics, Vol. 15, No. 2, 02.1993, p. 415-417.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Orphan Peak Analysis
T2 - A Novel Method for Detection of Point Mutations Using an Automated Fluorescence DNA Sequencer
AU - Hattori, Masahira
AU - Shibata, Asami
AU - Yoshioka, Katsuji
AU - Sakaki, Yoshiyuki
PY - 1993/2
Y1 - 1993/2
N2 - Automated DNA sequencers draw the four-base profiles of a sample with four different colors, but it is also possible to draw the profiles of a base-specific reaction of four different samples with four colors. PCR-amplified DNAs from four individuals were subjected to a single base-specific sequencing reaction and the products were applied to a set of four lanes of an automated DNA sequencer. A base substitution in an individual was clearly identified as an individual-specific peak with a color specific for the individual. In this way, we analyzed more than 50 individuals and identified several polymorphic base substitutions. The sensitivity of this method was high enough to allow detection of the mutation/polymorphism even if samples from several individuals were applied to one lane. Thus, our method is applicable to screening of a large number of samples in an automated manner.
AB - Automated DNA sequencers draw the four-base profiles of a sample with four different colors, but it is also possible to draw the profiles of a base-specific reaction of four different samples with four colors. PCR-amplified DNAs from four individuals were subjected to a single base-specific sequencing reaction and the products were applied to a set of four lanes of an automated DNA sequencer. A base substitution in an individual was clearly identified as an individual-specific peak with a color specific for the individual. In this way, we analyzed more than 50 individuals and identified several polymorphic base substitutions. The sensitivity of this method was high enough to allow detection of the mutation/polymorphism even if samples from several individuals were applied to one lane. Thus, our method is applicable to screening of a large number of samples in an automated manner.
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U2 - 10.1006/geno.1993.1077
DO - 10.1006/geno.1993.1077
M3 - Article
VL - 15
SP - 415
EP - 417
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 2
ER -