O2 binding and dissociation and ligand exchange reaction of O2 with CO in polymer composite films of hemoglobin

Sung Ick Park, Hiromi Sakai, Shinji Takeoka, Hiroyuki Nishide, Eishun Tsuchida

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A polymer composite film of hemoglobin (Hb-polymer film) was prepared by the casting of an Hb-polymer mixed solution (weight ratio of Hb to polymer is 1 to 1). The percentages of O2 and CO saturation of the Hb-dextran film were 46% and 70%, respectively. In the Hb solution, 100% saturation was observed for both Hgands, and a humidified Hb-dextran film also showed 100% saturation. Water molecules would provide flexibility to the matrix polymer and promote a structural change in the Hb from a tense state (T) to a relaxed state (R). Thus the ligand binding to the Hb in the polymer films was strongly affected by the degree of interaction of Hb with the matrix polymers and the physical properties of the polymers. Inositol hexaphosphate (IHP) worked as an allosteric effector even in the solid polymer film and lowered the oxygen affinity of Hb. The O2 transport through an Hb-polyethyleneimine (PEI) film with IHP showed the facilitated O2 transport in comparison with the film without IHP because of the high dissociation rate of O2 from Hb with IHP.

Original languageEnglish
Pages (from-to)366-370
Number of pages5
JournalPolymers for Advanced Technologies
Volume8
Issue number6
Publication statusPublished - 1997 Jun

Fingerprint

Phytic Acid
Hemoglobin
Composite films
Carbon Monoxide
Polymer films
Ion exchange
Hemoglobins
Ligands
Polymers
Dextran
Dextrans
Polymer matrix
Polyethyleneimine
Saturation (materials composition)
Casting
Physical properties
Oxygen
Molecules
Water

Keywords

  • Dextran
  • Facilitated transport
  • Hemoglobin
  • Polyethyleneimine
  • Polymer composite film

ASJC Scopus subject areas

  • Polymers and Plastics

Cite this

O2 binding and dissociation and ligand exchange reaction of O2 with CO in polymer composite films of hemoglobin. / Park, Sung Ick; Sakai, Hiromi; Takeoka, Shinji; Nishide, Hiroyuki; Tsuchida, Eishun.

In: Polymers for Advanced Technologies, Vol. 8, No. 6, 06.1997, p. 366-370.

Research output: Contribution to journalArticle

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AB - A polymer composite film of hemoglobin (Hb-polymer film) was prepared by the casting of an Hb-polymer mixed solution (weight ratio of Hb to polymer is 1 to 1). The percentages of O2 and CO saturation of the Hb-dextran film were 46% and 70%, respectively. In the Hb solution, 100% saturation was observed for both Hgands, and a humidified Hb-dextran film also showed 100% saturation. Water molecules would provide flexibility to the matrix polymer and promote a structural change in the Hb from a tense state (T) to a relaxed state (R). Thus the ligand binding to the Hb in the polymer films was strongly affected by the degree of interaction of Hb with the matrix polymers and the physical properties of the polymers. Inositol hexaphosphate (IHP) worked as an allosteric effector even in the solid polymer film and lowered the oxygen affinity of Hb. The O2 transport through an Hb-polyethyleneimine (PEI) film with IHP showed the facilitated O2 transport in comparison with the film without IHP because of the high dissociation rate of O2 from Hb with IHP.

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