Over-expression of putative transcriptional coactivator KELP interferes with Tomato mosaic virus cell-to-cell movement

Nobumitsu Sasaki, Takuya Ogata, Masakazu Deguchi, Shoko Nagai, Atsushi Tamai, Tetsuo Meshi, Shigeki Kawakami, Yuichiro Watanabe, Yasuhiko Matsushita, Hiroshi Nyunoya

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25 Citations (Scopus)

Abstract

Tomato mosaic virus (ToMV) encodes a movement protein (MP) that is necessary for virus cell-to-cell movement. We have demonstrated previously that KELP, a putative transcriptional coactivator of Arabidopsis thaliana, and its orthologue from Brassica campestris can bind to ToMV MP in vitro. In this study, we examined the effects of the transient over-expression of KELP on ToMV infection and the intracellular localization of MP in Nicotiana benthamiana, an experimental host of the virus. In co-bombardment experiments, the over-expression of KELP inhibited virus cell-to-cell movement. The N-terminal half of KELP (KELPdC), which had been shown to bind to MP, was sufficient for inhibition. Furthermore, the over-expression of KELP and KELPdC, both of which were co-localized with ToMV MP, led to a reduction in the plasmodesmal association of MP. In the absence of MP expression, KELP was localized in the nucleus and the cytoplasm by the localization signal in its N-terminal half. It was also shown that ToMV amplified normally in protoplasts prepared from leaf tissue that expressed KELP transiently. These results indicate that over-expressed KELP interacts with MP in vivo and exerts an inhibitory effect on MP function for virus cell-to-cell movement, but not on virus amplification in individual cells.

Original languageEnglish
Pages (from-to)161-173
Number of pages13
JournalMolecular Plant Pathology
Volume10
Issue number2
DOIs
Publication statusPublished - 2009 Mar 1
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Agronomy and Crop Science
  • Soil Science
  • Plant Science

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    Sasaki, N., Ogata, T., Deguchi, M., Nagai, S., Tamai, A., Meshi, T., Kawakami, S., Watanabe, Y., Matsushita, Y., & Nyunoya, H. (2009). Over-expression of putative transcriptional coactivator KELP interferes with Tomato mosaic virus cell-to-cell movement. Molecular Plant Pathology, 10(2), 161-173. https://doi.org/10.1111/j.1364-3703.2008.00517.x