Over-expression of putative transcriptional coactivator KELP interferes with Tomato mosaic virus cell-to-cell movement

Nobumitsu Sasaki, Takuya Ogata, Masakazu Deguchi, Shoko Nagai, Atsushi Tamai, Tetsuo Meshi, Shigeki Kawakami, Yuichiro Watanabe, Yasuhiko Matsushita, Hiroshi Nyunoya

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Tomato mosaic virus (ToMV) encodes a movement protein (MP) that is necessary for virus cell-to-cell movement. We have demonstrated previously that KELP, a putative transcriptional coactivator of Arabidopsis thaliana, and its orthologue from Brassica campestris can bind to ToMV MP in vitro. In this study, we examined the effects of the transient over-expression of KELP on ToMV infection and the intracellular localization of MP in Nicotiana benthamiana, an experimental host of the virus. In co-bombardment experiments, the over-expression of KELP inhibited virus cell-to-cell movement. The N-terminal half of KELP (KELPdC), which had been shown to bind to MP, was sufficient for inhibition. Furthermore, the over-expression of KELP and KELPdC, both of which were co-localized with ToMV MP, led to a reduction in the plasmodesmal association of MP. In the absence of MP expression, KELP was localized in the nucleus and the cytoplasm by the localization signal in its N-terminal half. It was also shown that ToMV amplified normally in protoplasts prepared from leaf tissue that expressed KELP transiently. These results indicate that over-expressed KELP interacts with MP in vivo and exerts an inhibitory effect on MP function for virus cell-to-cell movement, but not on virus amplification in individual cells.

Original languageEnglish
Pages (from-to)161-173
Number of pages13
JournalMolecular Plant Pathology
Volume10
Issue number2
DOIs
Publication statusPublished - 2009 Mar
Externally publishedYes

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Tomato mosaic virus
Mosaic Viruses
Lycopersicon esculentum
cell movement
Cell Movement
virus
protein
Proteins
viruses
proteins
Viruses
cells
Brassica rapa subsp. oleifera
mosaic
Nicotiana benthamiana
Protoplasts
Brassica
Virus Diseases
protoplasts
Arabidopsis

ASJC Scopus subject areas

  • Molecular Biology
  • Agronomy and Crop Science
  • Soil Science
  • Plant Science

Cite this

Over-expression of putative transcriptional coactivator KELP interferes with Tomato mosaic virus cell-to-cell movement. / Sasaki, Nobumitsu; Ogata, Takuya; Deguchi, Masakazu; Nagai, Shoko; Tamai, Atsushi; Meshi, Tetsuo; Kawakami, Shigeki; Watanabe, Yuichiro; Matsushita, Yasuhiko; Nyunoya, Hiroshi.

In: Molecular Plant Pathology, Vol. 10, No. 2, 03.2009, p. 161-173.

Research output: Contribution to journalArticle

Sasaki, N, Ogata, T, Deguchi, M, Nagai, S, Tamai, A, Meshi, T, Kawakami, S, Watanabe, Y, Matsushita, Y & Nyunoya, H 2009, 'Over-expression of putative transcriptional coactivator KELP interferes with Tomato mosaic virus cell-to-cell movement', Molecular Plant Pathology, vol. 10, no. 2, pp. 161-173. https://doi.org/10.1111/j.1364-3703.2008.00517.x
Sasaki, Nobumitsu ; Ogata, Takuya ; Deguchi, Masakazu ; Nagai, Shoko ; Tamai, Atsushi ; Meshi, Tetsuo ; Kawakami, Shigeki ; Watanabe, Yuichiro ; Matsushita, Yasuhiko ; Nyunoya, Hiroshi. / Over-expression of putative transcriptional coactivator KELP interferes with Tomato mosaic virus cell-to-cell movement. In: Molecular Plant Pathology. 2009 ; Vol. 10, No. 2. pp. 161-173.
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