Overexpression of a tobacco Dof transcription factor BBF1 stimulates the transcription of the Tobacco mosaic virus resistance gene N and defense-related responses including ROS production

Mayumi Takano, Md Ashraful Haquea, Shota Odaira, Keiko Nakata, Nobumitsu Sasaki, Hiroshi Nyunoya

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Dof proteins are known as plant-specific transcription factors. We previously reported that Dof proteins might be involved in expression of the tobacco resistance gene N for Tobacco mosaic virus (TMV). In N-carrying tobacco cultivars such as Samsun NN, a rapid upregulation of N transcription is induced by TMV infection, which is followed by the defense response called the hypersensitive response (HR). In this study, the role of a tobacco Dof protein BBF1 in N transcription was investigated. We cloned a BBF1 ORF cDNA of Samsun NN and confirmed that a full-length recombinant BBF1 could bind in vitro to DNA with a Dof binding core motif. The transient overexpression of BBF1 alone did not induce any HR but activated the endogenous N gene expression in Samsun NN. The N promoter activation by BBF1 overexpression was also confirmed in the N-lacking Samsun nn plant by using the exogenously introduced N regulatory sequence connected to a reporter gene. Additional experiments suggested that BBF1 overexpression enhanced not only ROS production but also the transcription activity of certain defense signaling and HR marker genes even without HR induction. Regarding subcellular localization, BBF1 fused with a fluorescent protein was predominantly localized in the nucleus. Based on these data, we discuss potential roles of BBF1 and other Dof proteins as transcription factors for defense responses. Dof proteins are known as plant-specific transcription factors. We previously reported that Dof proteins might be involved in expression of the tobacco resistance gene N for Tobacco mosaic virus (TMV). In N-carrying tobacco cultivars such as Samsun NN, a rapid upregulation of N transcription is induced by TMV infection, which is followed by the defense response called the hypersensitive response (HR). In this study, the role of a tobacco Dof protein BBF1 in N transcription was investigated. We cloned a BBF1 ORF cDNA of Samsun NN and confirmed that a full-length recombinant BBF1 could bind in vitro to DNA with a Dof binding core motif. The transient overexpression of BBF1 alone did not induce any HR but activated the endogenous N gene expression in Samsun NN. The N promoter activation by BBF1 overexpression was also confirmed in the N-lacking Samsun nn plant by using the exogenously introduced N regulatory sequence connected to a reporter gene. Additional experiments suggested that BBF1 overexpression enhanced not only ROS production but also the transcription activity of certain defense signaling and HR marker genes even without HR induction. Regarding subcellular localization, BBF1 fused with a fluorescent protein was predominantly localized in the nucleus. Based on these data, we discuss potential roles of BBF1 and other Dof proteins as transcription factors for defense responses.

Original languageEnglish
Pages (from-to)37-46
Number of pages10
JournalPlant Biotechnology
Volume30
Issue number1
DOIs
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

Tobacco Mosaic Virus
Tobacco mosaic virus
hypersensitive response
Tobacco
tobacco
Transcription Factors
transcription factors
transcription (genetics)
Genes
Proteins
genes
proteins
regulatory sequences
reporter genes
open reading frames
Virus Diseases
Reporter Genes
promoter regions
Open Reading Frames
Up-Regulation

Keywords

  • Dof transcription factor
  • HR
  • N gene
  • ROS
  • TMV

ASJC Scopus subject areas

  • Plant Science
  • Biotechnology
  • Agronomy and Crop Science

Cite this

Overexpression of a tobacco Dof transcription factor BBF1 stimulates the transcription of the Tobacco mosaic virus resistance gene N and defense-related responses including ROS production. / Takano, Mayumi; Haquea, Md Ashraful; Odaira, Shota; Nakata, Keiko; Sasaki, Nobumitsu; Nyunoya, Hiroshi.

In: Plant Biotechnology, Vol. 30, No. 1, 2013, p. 37-46.

Research output: Contribution to journalArticle

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abstract = "Dof proteins are known as plant-specific transcription factors. We previously reported that Dof proteins might be involved in expression of the tobacco resistance gene N for Tobacco mosaic virus (TMV). In N-carrying tobacco cultivars such as Samsun NN, a rapid upregulation of N transcription is induced by TMV infection, which is followed by the defense response called the hypersensitive response (HR). In this study, the role of a tobacco Dof protein BBF1 in N transcription was investigated. We cloned a BBF1 ORF cDNA of Samsun NN and confirmed that a full-length recombinant BBF1 could bind in vitro to DNA with a Dof binding core motif. The transient overexpression of BBF1 alone did not induce any HR but activated the endogenous N gene expression in Samsun NN. The N promoter activation by BBF1 overexpression was also confirmed in the N-lacking Samsun nn plant by using the exogenously introduced N regulatory sequence connected to a reporter gene. Additional experiments suggested that BBF1 overexpression enhanced not only ROS production but also the transcription activity of certain defense signaling and HR marker genes even without HR induction. Regarding subcellular localization, BBF1 fused with a fluorescent protein was predominantly localized in the nucleus. Based on these data, we discuss potential roles of BBF1 and other Dof proteins as transcription factors for defense responses. Dof proteins are known as plant-specific transcription factors. We previously reported that Dof proteins might be involved in expression of the tobacco resistance gene N for Tobacco mosaic virus (TMV). In N-carrying tobacco cultivars such as Samsun NN, a rapid upregulation of N transcription is induced by TMV infection, which is followed by the defense response called the hypersensitive response (HR). In this study, the role of a tobacco Dof protein BBF1 in N transcription was investigated. We cloned a BBF1 ORF cDNA of Samsun NN and confirmed that a full-length recombinant BBF1 could bind in vitro to DNA with a Dof binding core motif. The transient overexpression of BBF1 alone did not induce any HR but activated the endogenous N gene expression in Samsun NN. The N promoter activation by BBF1 overexpression was also confirmed in the N-lacking Samsun nn plant by using the exogenously introduced N regulatory sequence connected to a reporter gene. Additional experiments suggested that BBF1 overexpression enhanced not only ROS production but also the transcription activity of certain defense signaling and HR marker genes even without HR induction. Regarding subcellular localization, BBF1 fused with a fluorescent protein was predominantly localized in the nucleus. Based on these data, we discuss potential roles of BBF1 and other Dof proteins as transcription factors for defense responses.",
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AU - Sasaki, Nobumitsu

AU - Nyunoya, Hiroshi

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