Oxalic acid production by citric acid-producing Aspergillus niger overexpressing the oxaloacetate hydrolase gene oahA

Keiichi Kobayashi, Takasumi Hattori, Yuki Honda, Kotaro Kirimura

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    The filamentous fungus Aspergillus niger is used worldwide in the industrial production of citric acid. However, under specific cultivation conditions, citric acid-producing strains of A. niger accumulate oxalic acid as a by-product. Oxalic acid is used as a chelator, detergent, or tanning agent. Here, we sought to develop oxalic acid hyperproducers using A. niger as a host. To generate oxalic acid hyperproducers by metabolic engineering, transformants overexpressing the oahA gene, encoding oxaloacetate hydrolase (OAH; EC 3.7.1.1), were constructed in citric acid-producing A. niger WU-2223L as a host. The oxalic acid production capacity of this strain was examined by cultivation of EOAH-1 under conditions appropriate for oxalic acid production with 30 g/l glucose as a carbon source. Under all the cultivation conditions tested, the amount of oxalic acid produced by EOAH-1, a representative oahA-overexpressing transformant, exceeded that produced by A. niger WU-2223L. A. niger WU-2223L and EOAH-1 produced 15.6 and 28.9 g/l oxalic acid, respectively, during the 12-day cultivation period. The yield of oxalic acid for EOAH-1 was 64.2 % of the maximum theoretical yield. Our method for oxalic acid production gave the highest yield of any study reported to date. Therefore, we succeeded in generating oxalic acid hyperproducers by overexpressing a single gene, i.e., oahA, in citric acid-producing A. niger as a host.

    Original languageEnglish
    Pages (from-to)749-756
    Number of pages8
    JournalJournal of Industrial Microbiology and Biotechnology
    Volume41
    Issue number5
    DOIs
    Publication statusPublished - 2014

    Fingerprint

    oxaloacetase
    Oxalic Acid
    Hydrolases
    Oxalic acid
    Aspergillus niger
    Aspergillus
    Citric acid
    Citric Acid
    Genes
    Tanning
    Metabolic engineering
    Metabolic Engineering
    Gene encoding

    Keywords

    • Aspergillus niger
    • Citric acid
    • Metabolic engineering
    • Oxalic acid production
    • Oxaloacetate hydrolase

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology

    Cite this

    Oxalic acid production by citric acid-producing Aspergillus niger overexpressing the oxaloacetate hydrolase gene oahA. / Kobayashi, Keiichi; Hattori, Takasumi; Honda, Yuki; Kirimura, Kotaro.

    In: Journal of Industrial Microbiology and Biotechnology, Vol. 41, No. 5, 2014, p. 749-756.

    Research output: Contribution to journalArticle

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