Oxidative stress induced by intermittent exposure at a simulated altitude of 4000 m decreases mitochondrial superoxide dismutase content in soleus muscle of rats

Z. Radák, K. Lee, W. Choi, S. Sunoo, T. Kizaki, S. Oh-ishi, K. Suzuki, N. Taniguchi, H. Ohno, K. Asano

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The effects were examined of 6-month intermittent hypobaric (4000 m) exposure on the antioxidant enzyme systems in soleus and tibialis muscles of rats. At the end of the 6-month experimental exposure, the six rats in both the exposed group and the control group were sacrificed. Immunoreactive mitochondrial superoxide dismutase (Mn-SOD) contents were measured as well as the activities of antioxidant enzymes [Mn-SOD, cytosolic SOD (Cu,Zn-SOD), catalase (CAT), and glutathione peroxidase (GPX)]. Thiobarbituric acid-reactive substances (TBARS) were also determined as an indicator of lipid peroxidation. The high altitude exposure resulted in a marked increase in TBARS content in soleus muscle, suggesting increased levels of oxygen free radicals. Conversely, significant decreases in both Mn-SOD content and activity in solens muscle were oted affer exposure. Such trends were not noticed in tibialis muscle. On the other hand, no significant changes in Cu,Zn-SOD, CAT, or GPX were observed in either muscle. These results suggested that the increases in lipid peroxidation were most probably a result of decreased Mn-SOD function which was more depressed in oxidative than in glycolytic muscle.

Original languageEnglish
Pages (from-to)392-395
Number of pages4
JournalEuropean Journal of Applied Physiology and Occupational Physiology
Volume69
Issue number5
DOIs
Publication statusPublished - 1994 Sep 1
Externally publishedYes

Keywords

  • Antioxidant enzymes
  • Fibre type
  • Intermittent exposure to high altitude
  • Lipid peroxidation
  • Mitochondrial superoxide dismutase

ASJC Scopus subject areas

  • Physiology
  • Public Health, Environmental and Occupational Health

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