Oxidative stress induced by intermittent exposure at a simulated altitude of 4000 m decreases mitochondrial superoxide dismutase content in soleus muscle of rats

Zsolt Radak, K. Lee, W. Choi, S. Sunoo, T. Kizaki, S. Oh-ishi, K. Suzuki, N. Taniguchi, H. Ohno, K. Asano

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The effects were examined of 6-month intermittent hypobaric (4000 m) exposure on the antioxidant enzyme systems in soleus and tibialis muscles of rats. At the end of the 6-month experimental exposure, the six rats in both the exposed group and the control group were sacrificed. Immunoreactive mitochondrial superoxide dismutase (Mn-SOD) contents were measured as well as the activities of antioxidant enzymes [Mn-SOD, cytosolic SOD (Cu,Zn-SOD), catalase (CAT), and glutathione peroxidase (GPX)]. Thiobarbituric acid-reactive substances (TBARS) were also determined as an indicator of lipid peroxidation. The high altitude exposure resulted in a marked increase in TBARS content in soleus muscle, suggesting increased levels of oxygen free radicals. Conversely, significant decreases in both Mn-SOD content and activity in solens muscle were oted affer exposure. Such trends were not noticed in tibialis muscle. On the other hand, no significant changes in Cu,Zn-SOD, CAT, or GPX were observed in either muscle. These results suggested that the increases in lipid peroxidation were most probably a result of decreased Mn-SOD function which was more depressed in oxidative than in glycolytic muscle.

Original languageEnglish
Pages (from-to)392-395
Number of pages4
JournalEuropean Journal of Applied Physiology and Occupational Physiology
Volume69
Issue number5
DOIs
Publication statusPublished - 1994 Sep 1
Externally publishedYes

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Superoxide Dismutase
Skeletal Muscle
Oxidative Stress
Muscles
Thiobarbituric Acid Reactive Substances
Glutathione Peroxidase
Catalase
Lipid Peroxidation
Antioxidants
Enzymes
Free Radicals
Reactive Oxygen Species
Control Groups

Keywords

  • Antioxidant enzymes
  • Fibre type
  • Intermittent exposure to high altitude
  • Lipid peroxidation
  • Mitochondrial superoxide dismutase

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Physiology

Cite this

Oxidative stress induced by intermittent exposure at a simulated altitude of 4000 m decreases mitochondrial superoxide dismutase content in soleus muscle of rats. / Radak, Zsolt; Lee, K.; Choi, W.; Sunoo, S.; Kizaki, T.; Oh-ishi, S.; Suzuki, K.; Taniguchi, N.; Ohno, H.; Asano, K.

In: European Journal of Applied Physiology and Occupational Physiology, Vol. 69, No. 5, 01.09.1994, p. 392-395.

Research output: Contribution to journalArticle

Radak, Zsolt ; Lee, K. ; Choi, W. ; Sunoo, S. ; Kizaki, T. ; Oh-ishi, S. ; Suzuki, K. ; Taniguchi, N. ; Ohno, H. ; Asano, K. / Oxidative stress induced by intermittent exposure at a simulated altitude of 4000 m decreases mitochondrial superoxide dismutase content in soleus muscle of rats. In: European Journal of Applied Physiology and Occupational Physiology. 1994 ; Vol. 69, No. 5. pp. 392-395.
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