Oxidative/heat stress enhanced production of chitosanase from Streptomyces griseus cells through its interaction with liposome

Kien Xuan Ngo, Hiroshi Umakoshi, Haruyuki Ishii, Huong Thi Bui, Toshinori Shimanouchi, Ryoichi Kuboi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The effective production and secretion of chitosanase from S. griseus cells, in the presence of hydrogen peroxide, were studied by the treatment of the cells with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) liposomes under heat condition. The variation of the conformation and activity of the chitosanase caused by the interaction of liposomes with target chitosanase under stress condition was systematically investigated by using circular dichroism (CD) spectra and dielectric dispersion analysis (DDA). The effect of the oxidative stress (hydrogen peroxide) on the lipid and protein peroxide of cell membrane of S. griseus pretreated with and without liposomes under heat stress at 41 °C was further carried out. The possible utilization of membrane-membrane interaction between liposomes and cell membrane induced by the heat treatment was further investigated to enhance the production of chitosanase from S. griseus under oxidative stress condition.

Original languageEnglish
Pages (from-to)471-476
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume108
Issue number6
DOIs
Publication statusPublished - 2009 Dec
Externally publishedYes

Fingerprint

chitosanase
Streptomyces griseus
Liposomes
Oxidative Stress
Hot Temperature
Oxidative stress
Cell membranes
Hydrogen peroxide
Hydrogen Peroxide
Membranes
Lipid Peroxides
Peroxides
Dichroism
Circular Dichroism
Lipids
Conformations
Membrane Proteins
Permittivity
Heat treatment
Cells

Keywords

  • Chitosanase
  • Liposome
  • Membrane stress biotechnology
  • Oxidative stress
  • Streptomyces griseus

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Oxidative/heat stress enhanced production of chitosanase from Streptomyces griseus cells through its interaction with liposome. / Ngo, Kien Xuan; Umakoshi, Hiroshi; Ishii, Haruyuki; Bui, Huong Thi; Shimanouchi, Toshinori; Kuboi, Ryoichi.

In: Journal of Bioscience and Bioengineering, Vol. 108, No. 6, 12.2009, p. 471-476.

Research output: Contribution to journalArticle

Ngo, Kien Xuan ; Umakoshi, Hiroshi ; Ishii, Haruyuki ; Bui, Huong Thi ; Shimanouchi, Toshinori ; Kuboi, Ryoichi. / Oxidative/heat stress enhanced production of chitosanase from Streptomyces griseus cells through its interaction with liposome. In: Journal of Bioscience and Bioengineering. 2009 ; Vol. 108, No. 6. pp. 471-476.
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