Oxidative/heat stress enhanced production of chitosanase from Streptomyces griseus cells through its interaction with liposome

Kien Xuan Ngo; Hiroshi Umakoshi; Haruyuki Ishii; Huong Thi Bui; Toshinori Shimanouchi; Ryoichi Kuboi

doi:10.1016/j.jbiosc.2009.06.010

Oxidative/heat stress enhanced production of chitosanase from Streptomyces griseus cells through its interaction with liposome

Kien Xuan Ngo, Hiroshi Umakoshi, Haruyuki Ishii, Huong Thi Bui, Toshinori Shimanouchi, Ryoichi Kuboi

Research output: Contribution to journal › Article

3 Citations (Scopus)

Abstract

The effective production and secretion of chitosanase from S. griseus cells, in the presence of hydrogen peroxide, were studied by the treatment of the cells with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) liposomes under heat condition. The variation of the conformation and activity of the chitosanase caused by the interaction of liposomes with target chitosanase under stress condition was systematically investigated by using circular dichroism (CD) spectra and dielectric dispersion analysis (DDA). The effect of the oxidative stress (hydrogen peroxide) on the lipid and protein peroxide of cell membrane of S. griseus pretreated with and without liposomes under heat stress at 41 °C was further carried out. The possible utilization of membrane-membrane interaction between liposomes and cell membrane induced by the heat treatment was further investigated to enhance the production of chitosanase from S. griseus under oxidative stress condition.

Original language	English
Pages (from-to)	471-476
Number of pages	6
Journal	Journal of Bioscience and Bioengineering
Volume	108
Issue number	6
DOIs	https://doi.org/10.1016/j.jbiosc.2009.06.010
Publication status	Published - 2009 Dec
Externally published	Yes

Keywords

Chitosanase
Liposome
Membrane stress biotechnology
Oxidative stress
Streptomyces griseus

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology
Bioengineering

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Ngo, K. X., Umakoshi, H., Ishii, H., Bui, H. T., Shimanouchi, T., & Kuboi, R. (2009). Oxidative/heat stress enhanced production of chitosanase from Streptomyces griseus cells through its interaction with liposome. Journal of Bioscience and Bioengineering, 108(6), 471-476. https://doi.org/10.1016/j.jbiosc.2009.06.010

Oxidative/heat stress enhanced production of chitosanase from Streptomyces griseus cells through its interaction with liposome. / Ngo, Kien Xuan; Umakoshi, Hiroshi; Ishii, Haruyuki; Bui, Huong Thi; Shimanouchi, Toshinori; Kuboi, Ryoichi.

In: Journal of Bioscience and Bioengineering, Vol. 108, No. 6, 12.2009, p. 471-476.

Research output: Contribution to journal › Article

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title = "Oxidative/heat stress enhanced production of chitosanase from Streptomyces griseus cells through its interaction with liposome",

abstract = "The effective production and secretion of chitosanase from S. griseus cells, in the presence of hydrogen peroxide, were studied by the treatment of the cells with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) liposomes under heat condition. The variation of the conformation and activity of the chitosanase caused by the interaction of liposomes with target chitosanase under stress condition was systematically investigated by using circular dichroism (CD) spectra and dielectric dispersion analysis (DDA). The effect of the oxidative stress (hydrogen peroxide) on the lipid and protein peroxide of cell membrane of S. griseus pretreated with and without liposomes under heat stress at 41 °C was further carried out. The possible utilization of membrane-membrane interaction between liposomes and cell membrane induced by the heat treatment was further investigated to enhance the production of chitosanase from S. griseus under oxidative stress condition.",

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AU - Shimanouchi, Toshinori

AU - Kuboi, Ryoichi

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