Abstract
The effective production and secretion of chitosanase from S. griseus cells, in the presence of hydrogen peroxide, were studied by the treatment of the cells with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) liposomes under heat condition. The variation of the conformation and activity of the chitosanase caused by the interaction of liposomes with target chitosanase under stress condition was systematically investigated by using circular dichroism (CD) spectra and dielectric dispersion analysis (DDA). The effect of the oxidative stress (hydrogen peroxide) on the lipid and protein peroxide of cell membrane of S. griseus pretreated with and without liposomes under heat stress at 41 °C was further carried out. The possible utilization of membrane-membrane interaction between liposomes and cell membrane induced by the heat treatment was further investigated to enhance the production of chitosanase from S. griseus under oxidative stress condition.
| Original language | English |
|---|---|
| Pages (from-to) | 471-476 |
| Number of pages | 6 |
| Journal | Journal of Bioscience and Bioengineering |
| Volume | 108 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2009 Dec |
| Externally published | Yes |
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Keywords
- Chitosanase
- Liposome
- Membrane stress biotechnology
- Oxidative stress
- Streptomyces griseus
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
- Bioengineering
Cite this
Oxidative/heat stress enhanced production of chitosanase from Streptomyces griseus cells through its interaction with liposome. / Ngo, Kien Xuan; Umakoshi, Hiroshi; Ishii, Haruyuki; Bui, Huong Thi; Shimanouchi, Toshinori; Kuboi, Ryoichi.
In: Journal of Bioscience and Bioengineering, Vol. 108, No. 6, 12.2009, p. 471-476.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Oxidative/heat stress enhanced production of chitosanase from Streptomyces griseus cells through its interaction with liposome
AU - Ngo, Kien Xuan
AU - Umakoshi, Hiroshi
AU - Ishii, Haruyuki
AU - Bui, Huong Thi
AU - Shimanouchi, Toshinori
AU - Kuboi, Ryoichi
PY - 2009/12
Y1 - 2009/12
N2 - The effective production and secretion of chitosanase from S. griseus cells, in the presence of hydrogen peroxide, were studied by the treatment of the cells with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) liposomes under heat condition. The variation of the conformation and activity of the chitosanase caused by the interaction of liposomes with target chitosanase under stress condition was systematically investigated by using circular dichroism (CD) spectra and dielectric dispersion analysis (DDA). The effect of the oxidative stress (hydrogen peroxide) on the lipid and protein peroxide of cell membrane of S. griseus pretreated with and without liposomes under heat stress at 41 °C was further carried out. The possible utilization of membrane-membrane interaction between liposomes and cell membrane induced by the heat treatment was further investigated to enhance the production of chitosanase from S. griseus under oxidative stress condition.
AB - The effective production and secretion of chitosanase from S. griseus cells, in the presence of hydrogen peroxide, were studied by the treatment of the cells with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) liposomes under heat condition. The variation of the conformation and activity of the chitosanase caused by the interaction of liposomes with target chitosanase under stress condition was systematically investigated by using circular dichroism (CD) spectra and dielectric dispersion analysis (DDA). The effect of the oxidative stress (hydrogen peroxide) on the lipid and protein peroxide of cell membrane of S. griseus pretreated with and without liposomes under heat stress at 41 °C was further carried out. The possible utilization of membrane-membrane interaction between liposomes and cell membrane induced by the heat treatment was further investigated to enhance the production of chitosanase from S. griseus under oxidative stress condition.
KW - Chitosanase
KW - Liposome
KW - Membrane stress biotechnology
KW - Oxidative stress
KW - Streptomyces griseus
UR - http://www.scopus.com/inward/record.url?scp=70449368214&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70449368214&partnerID=8YFLogxK
U2 - 10.1016/j.jbiosc.2009.06.010
DO - 10.1016/j.jbiosc.2009.06.010
M3 - Article
C2 - 19914578
AN - SCOPUS:70449368214
VL - 108
SP - 471
EP - 476
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 6
ER -