Oxidized guanine base lesions function in 8-oxoguanine DNA glycosylase-1-mediated epigenetic regulation of nuclear factor κB-driven gene expression

Lang Pan, Bing Zhu, Wenjing Hao, Xianlu Zeng, Spiros A. Vlahopoulos, Tapas K. Hazra, Muralidhar L. Hegde, Zsolt Radak, Attila Bacsi, Allan R. Brasier, Xueqing Ba, Istvan Boldogh

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

A large percentage of redox-responsive gene promoters contain evolutionarily conserved guanine-rich clusters; guanines are the bases most susceptible to oxidative modification(s). Consequently, 7,8-dihydro-8-oxoguanine (8-oxoG) is one of the most abundant base lesions in promoters and is primarily repaired via the 8-oxoguanine DNA glycosylase-1 (OOG1)-initiated base excision repair pathway. In view of a prompt cellular response to oxidative challenge, we hypothesized that the 8-oxoG lesion and the cognate repair protein OGG1 are utilized in transcriptional gene activation. Here, we document TNFα-induced enrichment of both 8-oxoG and OGG1 in promoters of pro-inflammatory genes, which precedes interaction of NF-κB with its DNA-binding motif. OGG1 bound to 8-oxoG upstream from the NF-κB motif increased its DNA occupancy by promoting an on-rate of both homodimeric and heterodimeric forms of NF-κB. OGG1 depletion decreased both NF-κB binding and gene expression, whereas Nei-like glycosylase-1 and -2 had a marginal effect. These results are the first to document a novel paradigm wherein the DNA repair protein OGG1 bound to its substrate is coupled to DNA occupancy of NF-κB and functions in epigenetic regulation of gene expression.

Original languageEnglish
Pages (from-to)25553-25566
Number of pages14
JournalJournal of Biological Chemistry
Volume291
Issue number49
DOIs
Publication statusPublished - 2016 Dec 2
Externally publishedYes

Fingerprint

DNA Glycosylases
Guanine
Epigenomics
Gene expression
DNA Repair
Transcriptional Activation
Gene Expression
Nucleotide Motifs
Repair
Genes
DNA
Gene Expression Regulation
Oxidation-Reduction
Proteins
Chemical activation
8-hydroxyguanine
Substrates
7,8-dihydro-8-oxoguanine

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Oxidized guanine base lesions function in 8-oxoguanine DNA glycosylase-1-mediated epigenetic regulation of nuclear factor κB-driven gene expression. / Pan, Lang; Zhu, Bing; Hao, Wenjing; Zeng, Xianlu; Vlahopoulos, Spiros A.; Hazra, Tapas K.; Hegde, Muralidhar L.; Radak, Zsolt; Bacsi, Attila; Brasier, Allan R.; Ba, Xueqing; Boldogh, Istvan.

In: Journal of Biological Chemistry, Vol. 291, No. 49, 02.12.2016, p. 25553-25566.

Research output: Contribution to journalArticle

Pan, L, Zhu, B, Hao, W, Zeng, X, Vlahopoulos, SA, Hazra, TK, Hegde, ML, Radak, Z, Bacsi, A, Brasier, AR, Ba, X & Boldogh, I 2016, 'Oxidized guanine base lesions function in 8-oxoguanine DNA glycosylase-1-mediated epigenetic regulation of nuclear factor κB-driven gene expression', Journal of Biological Chemistry, vol. 291, no. 49, pp. 25553-25566. https://doi.org/10.1074/jbc.M116.751453
Pan, Lang ; Zhu, Bing ; Hao, Wenjing ; Zeng, Xianlu ; Vlahopoulos, Spiros A. ; Hazra, Tapas K. ; Hegde, Muralidhar L. ; Radak, Zsolt ; Bacsi, Attila ; Brasier, Allan R. ; Ba, Xueqing ; Boldogh, Istvan. / Oxidized guanine base lesions function in 8-oxoguanine DNA glycosylase-1-mediated epigenetic regulation of nuclear factor κB-driven gene expression. In: Journal of Biological Chemistry. 2016 ; Vol. 291, No. 49. pp. 25553-25566.
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