Oxygen transport by low and normal oxygen affinity hemoglobin vesicles in extreme hemodilution

Pedro Cabrales, Hiromi Sakai, Amy G. Tsai, Shinji Takeoka, Eishun Tsuchida, Marcos Intaglietta

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    Abstract

    The oxygen transport capacity of phospholipid vesicles encapsulating purified Hb (HbV) produced with a Po2 at which Hb is 50% saturated (P50) of 8 (HbV8) and 29 mmHg (HbV29) was investigated in the hamster chamber window model by using microvascular measurements to determine oxygen delivery during extreme hemodilution. Two isovolemic hemodilution steps were performed with 5% recombinant albumin (rHSA) until Hct was 35% of baseline. Isovolemic exchange was continued using HbV suspended in rHSA solution to a total [Hb] of 5.7 g/dl in blood. P50 was modified by coencapsulating pyridoxal 5′-phosphate. Final Hct was 11% for the HbV groups, with a plasma [Hb] of 2.1 ± 0.1 g/dl after exchange with HbV8 or HbV29. A reference group was hemodiluted to Hct 11% with only rHSA. All groups showed stable blood pressure and heart rate. Arterial oxygen tensions were significantly higher than baseline for the HbV groups and the rHSA group and significantly lower for the HbV groups compared with the rHSA group. Blood pressure was significantly higher for the HbV 8 group compared with the HbV29 group. Arteriolar and venular blood flows were significantly higher than baseline for the HbV groups. Microvascular oxygen delivery and extraction were similar for the HbV groups but lower for the rHSA group (P < 0.05). Venular and tissue Po2 were statistically higher for the HbV8 vs. the HbV29 and rHSA groups (P < 0.05). Improved tissue Po2 is obtained when red blood cells deliver oxygen in combination with a high- rather than low-affinity oxygen carrier.

    Original languageEnglish
    JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
    Volume288
    Issue number4 57-4
    DOIs
    Publication statusPublished - 2005 Apr

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    Keywords

    • Blood substitutes
    • Hemoglobin oxygen affinity
    • Oxygen-carrying capacity
    • Tissue oxygen

    ASJC Scopus subject areas

    • Physiology

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