Pacific Ocean and Japan Sea ecotypes of Japanese beech (Fagus crenata) differ in photosystem responses to continuous high light

Jun Ya Yamazaki, Etsuko Yoda, Ayako Takahashi, Kintake Sonoike, Emiko Maruta

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Two ecotypes of Japanese beech (Fagus crenata Blume), the Pacific Ocean type (PAO) and the Japan Sea type (JAS), show different responses to high solar irradiance. When PAO and JAS saplings were grown in continuous high-light (H), leaves of JAS became pale green. To elucidate this phenomenon, we investigated in vivo photochemistry based on pigment concentrations of Photosystem (PS) I and PS II and Western blot analysis. In JAS-H leaves, the amount of D1-protein decreased, resulting in decreases in the maximal quantum yield of PS II (F v/Fm) and electron transport rate, whereas PAO-H leaves maintained high activities. The PS I photochemistry determined by measurement of P-700 photo-oxidation showed that the intersystem electron pool size was 1.4 times greater in JAS-H leaves than in PAO-H leaves. Furthermore, the re-reduction kinetics of P-700+ showed that cyclic electron transport around PS I was 1.2 times faster in PAO-H leaves than in JAS-H leaves. Analysis of the area over the fluorescence induction kinetics indicated that the relative abundance of the PS IIα center increased in PAO-H leaves, whereas JAS leaves were observed to have low acclimation capacity to high light. These results demonstrate that PAO leaves possess acclimation mechanisms to continuous high light, whereas JAS leaves are more vulnerable to continuous high light, resulting in reduced leaf longevity owing to photoinhibition caused by increases in the intersystem electron pool size and suppression of photochemistry at the level of PS I and PS II.

Original languageEnglish
Pages (from-to)961-968
Number of pages8
JournalTree Physiology
Volume27
Issue number7
DOIs
Publication statusPublished - 2007 Jul
Externally publishedYes

Keywords

  • Chlorophyll a fluorescence
  • Intersystem electron pool size
  • P-700 photo-oxidation kinetics
  • Photoinhibition
  • Photosystem I
  • Photosystem II

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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