Paired pulse ratio analysis of insulin-induced synaptic plasticity in the snail brain

Jun Murakami, Ryuichi Okada, Yutaka Fujito, Manabu Sakakibara, Ken Lukowiak, Etsuro Ito

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Insulin's action in the brain can directly alter cognitive functioning. We have recently shown that molluscan insulin-related peptides are upregulated following a conditioned taste aversion (CTA) training procedure. In addition, when mammalian insulin is superfused over the isolated Lymnaea stagnalis central nervous system, it elicits long-term synaptic enhancement at the monosynaptic connection between the cerebral giant cell and the buccal 1 (B1) motor neuron. This synaptic enhancement is thought to be a neural correlate of CTA. Here, we examined whether the observed changes in synaptic plasticity were the result of presynaptic and/or postsynaptic alterations using the paired pulse procedure. The paired pulse ratio was unaltered following insulin application, suggesting that insulin's effects on synaptic plasticity are mediated postsynaptically in the B1 motor neuron. Thus, it was suggested that postsynaptic changes need to be considered when insulin's actions on synaptic plasticity are examined.

Original languageEnglish
Pages (from-to)1771-1773
Number of pages3
JournalJournal of Experimental Biology
Volume216
Issue number10
DOIs
Publication statusPublished - 2013 May
Externally publishedYes

Fingerprint

Neuronal Plasticity
Snails
snail
plasticity
snails
brain
insulin
Cheek
Motor Neurons
Insulin
Brain
Lymnaea
Giant Cells
nervous system
motor neurons
peptide
Central Nervous System
Lymnaea stagnalis
Peptides
giant cells

Keywords

  • Conditioned taste aversion
  • Insulin
  • Lymnaea
  • Paired pulse ratio
  • Synaptic plasticity

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Physiology
  • Insect Science
  • Aquatic Science

Cite this

Paired pulse ratio analysis of insulin-induced synaptic plasticity in the snail brain. / Murakami, Jun; Okada, Ryuichi; Fujito, Yutaka; Sakakibara, Manabu; Lukowiak, Ken; Ito, Etsuro.

In: Journal of Experimental Biology, Vol. 216, No. 10, 05.2013, p. 1771-1773.

Research output: Contribution to journalArticle

Murakami, J, Okada, R, Fujito, Y, Sakakibara, M, Lukowiak, K & Ito, E 2013, 'Paired pulse ratio analysis of insulin-induced synaptic plasticity in the snail brain', Journal of Experimental Biology, vol. 216, no. 10, pp. 1771-1773. https://doi.org/10.1242/jeb.083469
Murakami, Jun ; Okada, Ryuichi ; Fujito, Yutaka ; Sakakibara, Manabu ; Lukowiak, Ken ; Ito, Etsuro. / Paired pulse ratio analysis of insulin-induced synaptic plasticity in the snail brain. In: Journal of Experimental Biology. 2013 ; Vol. 216, No. 10. pp. 1771-1773.
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