PAL31, a novel nuclear protein, expressed in the developing brain

Hideki Mutai, Yasushi Toyoshima, Weiyong Sun, Naka Hattori, Satoshi Tanaka, Kunio Shiota

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We cloned a cDNA encoding a novel protein (PAL31) predominantly expressed in the fetal rat brain by differential display. PAL31 contains leucine-rich repeat domains, a highly acidic region and a putative nuclear localization signal. PAL31 has 50-70% similarity to SSP29, APRIL, LANP, PHAP I, and PP32. Expression of PAL31 mRNA in the brain was high during the fetal period and decreased after birth. Immunohistochemical studies showed that PAL31 is expressed in the entire embryonic brain, whereas in the adult brain its expression is restricted to the subventricular zone where there are neural progenitor cells. It was also revealed that PAL31 is colocalized with PCNA in the nucleus, indicating that the PAL31 expression is developmentally regulated. Considering the primary structure of PAL31 and its spatiotemporal expression pattern, PAL31 is a novel nuclear protein related to the development of the brain through the proliferation of neuronal cells. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)427-433
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume274
Issue number2
DOIs
Publication statusPublished - 2000 Aug 2
Externally publishedYes

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Keywords

  • Brain
  • Differential display
  • Nuclear protein
  • PAL31
  • Rat

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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