PAL31, a nuclear protein required for progression to the S phase

Weiyong Sun, Naka Hattori, Hideki Mutai, Yasushi Toyoshima, Hiromichi Kimura, Satoshi Tanaka, Kunio Shiota

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

PAL31 is a nuclear protein expressed by various cell types. In the present study, the expression and function of PAL31 were examined in the cytokine-regulated growth of T and B cell lines. Treatment of the cells with mitogens [ovine PRL, recombinant rat placental lactogen-I (PL-I) and human IL-3] caused a dose-dependent increase in the expression of PAL31 mRNA in the PRL-dependent cell line Nb2, and IL-3 dependent cell line BaF3. A time-course study on synchronized Nb2 cells revealed that the expression of PAL31 is specific to the late G1 and S phases. Immunocytological studies revealed that PAL31 accumulates in the nuclei at the S phase. Furthermore, the antisense oligonucleotide for PAL31 severely inhibited the proliferation of Nb2 cells by inhibiting cells progressing to the S phase. Thus, PAL31 is a nuclear protein associated with cell cycle progression.

Original languageEnglish
Pages (from-to)1048-1054
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume280
Issue number4
DOIs
Publication statusPublished - 2001
Externally publishedYes

Keywords

  • Antisense oligonucleotide
  • Cell cycle
  • Cell proliferation
  • IL.3
  • PAL31
  • PL-I
  • Prolactin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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