PAL31 expression in rat trophoblast giant cells

Mayumi Oda, Weiyong Sun, Naka Hattori, Satoshi Tanaka, Kunio Shiota

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

PAL31 is a proliferation-related acidic nuclear protein that belongs to the leucine-rich protein family and is expressed cell-cycle-dependently. Trophoblasts differentiate into the trophoblast giant cells (TGCs) through the unusual type of cell cycle, namely endoreduplication. In the present study, we investigated the spatiotemporal pattern of PAL31 expression in rat placenta and Rcho-1 cell line. The PAL31 mRNA concentration varied in different areas of the placenta, and was barely detectable in the TGC layer. In Rcho-1 cells, although the level of PAL31 mRNA decreased dramatically during differentiation, PAL31 was detected even after differentiation. The site of intranuclear localization of PAL31 mostly overlapped with that of PCNA in the undifferentiated Rcho-1 cells, while they were not overlapped in differentiated cells. Thus, the subcellular localization of PAL31 in Rcho-1 cells significantly changed, and loss of cell cycle dependency after differentiation was noted. PAL31 is suggested to play a role in the endoreduplication distinct from the usual DNA duplication.

Original languageEnglish
Pages (from-to)721-726
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume287
Issue number3
DOIs
Publication statusPublished - 2001 Sep 28
Externally publishedYes

Fingerprint

Trophoblasts
Giant Cells
Rats
Cells
Endoreduplication
Cell Cycle
Placenta
Messenger RNA
Proliferating Cell Nuclear Antigen
Nuclear Proteins
Leucine
Cell Line
DNA
Proteins

Keywords

  • Cell cycle
  • Cell proliferation
  • DNA replication
  • Endoreduplication
  • PAL31
  • PCNA
  • Placenta
  • Pregnancy
  • Rcho-1
  • Trophoblast

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

PAL31 expression in rat trophoblast giant cells. / Oda, Mayumi; Sun, Weiyong; Hattori, Naka; Tanaka, Satoshi; Shiota, Kunio.

In: Biochemical and Biophysical Research Communications, Vol. 287, No. 3, 28.09.2001, p. 721-726.

Research output: Contribution to journalArticle

Oda, M, Sun, W, Hattori, N, Tanaka, S & Shiota, K 2001, 'PAL31 expression in rat trophoblast giant cells', Biochemical and Biophysical Research Communications, vol. 287, no. 3, pp. 721-726. https://doi.org/10.1006/bbrc.2001.5646
Oda, Mayumi ; Sun, Weiyong ; Hattori, Naka ; Tanaka, Satoshi ; Shiota, Kunio. / PAL31 expression in rat trophoblast giant cells. In: Biochemical and Biophysical Research Communications. 2001 ; Vol. 287, No. 3. pp. 721-726.
@article{7a2f9b064de941a7a0d39dec64808f50,
title = "PAL31 expression in rat trophoblast giant cells",
abstract = "PAL31 is a proliferation-related acidic nuclear protein that belongs to the leucine-rich protein family and is expressed cell-cycle-dependently. Trophoblasts differentiate into the trophoblast giant cells (TGCs) through the unusual type of cell cycle, namely endoreduplication. In the present study, we investigated the spatiotemporal pattern of PAL31 expression in rat placenta and Rcho-1 cell line. The PAL31 mRNA concentration varied in different areas of the placenta, and was barely detectable in the TGC layer. In Rcho-1 cells, although the level of PAL31 mRNA decreased dramatically during differentiation, PAL31 was detected even after differentiation. The site of intranuclear localization of PAL31 mostly overlapped with that of PCNA in the undifferentiated Rcho-1 cells, while they were not overlapped in differentiated cells. Thus, the subcellular localization of PAL31 in Rcho-1 cells significantly changed, and loss of cell cycle dependency after differentiation was noted. PAL31 is suggested to play a role in the endoreduplication distinct from the usual DNA duplication.",
keywords = "Cell cycle, Cell proliferation, DNA replication, Endoreduplication, PAL31, PCNA, Placenta, Pregnancy, Rcho-1, Trophoblast",
author = "Mayumi Oda and Weiyong Sun and Naka Hattori and Satoshi Tanaka and Kunio Shiota",
year = "2001",
month = "9",
day = "28",
doi = "10.1006/bbrc.2001.5646",
language = "English",
volume = "287",
pages = "721--726",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - PAL31 expression in rat trophoblast giant cells

AU - Oda, Mayumi

AU - Sun, Weiyong

AU - Hattori, Naka

AU - Tanaka, Satoshi

AU - Shiota, Kunio

PY - 2001/9/28

Y1 - 2001/9/28

N2 - PAL31 is a proliferation-related acidic nuclear protein that belongs to the leucine-rich protein family and is expressed cell-cycle-dependently. Trophoblasts differentiate into the trophoblast giant cells (TGCs) through the unusual type of cell cycle, namely endoreduplication. In the present study, we investigated the spatiotemporal pattern of PAL31 expression in rat placenta and Rcho-1 cell line. The PAL31 mRNA concentration varied in different areas of the placenta, and was barely detectable in the TGC layer. In Rcho-1 cells, although the level of PAL31 mRNA decreased dramatically during differentiation, PAL31 was detected even after differentiation. The site of intranuclear localization of PAL31 mostly overlapped with that of PCNA in the undifferentiated Rcho-1 cells, while they were not overlapped in differentiated cells. Thus, the subcellular localization of PAL31 in Rcho-1 cells significantly changed, and loss of cell cycle dependency after differentiation was noted. PAL31 is suggested to play a role in the endoreduplication distinct from the usual DNA duplication.

AB - PAL31 is a proliferation-related acidic nuclear protein that belongs to the leucine-rich protein family and is expressed cell-cycle-dependently. Trophoblasts differentiate into the trophoblast giant cells (TGCs) through the unusual type of cell cycle, namely endoreduplication. In the present study, we investigated the spatiotemporal pattern of PAL31 expression in rat placenta and Rcho-1 cell line. The PAL31 mRNA concentration varied in different areas of the placenta, and was barely detectable in the TGC layer. In Rcho-1 cells, although the level of PAL31 mRNA decreased dramatically during differentiation, PAL31 was detected even after differentiation. The site of intranuclear localization of PAL31 mostly overlapped with that of PCNA in the undifferentiated Rcho-1 cells, while they were not overlapped in differentiated cells. Thus, the subcellular localization of PAL31 in Rcho-1 cells significantly changed, and loss of cell cycle dependency after differentiation was noted. PAL31 is suggested to play a role in the endoreduplication distinct from the usual DNA duplication.

KW - Cell cycle

KW - Cell proliferation

KW - DNA replication

KW - Endoreduplication

KW - PAL31

KW - PCNA

KW - Placenta

KW - Pregnancy

KW - Rcho-1

KW - Trophoblast

UR - http://www.scopus.com/inward/record.url?scp=0035964746&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035964746&partnerID=8YFLogxK

U2 - 10.1006/bbrc.2001.5646

DO - 10.1006/bbrc.2001.5646

M3 - Article

C2 - 11563855

AN - SCOPUS:0035964746

VL - 287

SP - 721

EP - 726

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -