Abstract
A parallel and passive (without any actuators or electrodes) cell distribution method for a cellular diagnostic microwell array was developed. This method can be used to simultaneously distribute cells evenly to arrayed microwells only by introducing cell suspensions into the inlet. To regulate the dispersion of the number of distributed cells into each microwell, we conceived a novel concept, the self-regulating pinched flow. The self-regulating pinched flow is realized in a functional microchannel, which consists of center micropillar colonnades and side channels. Computational fluid dynamics (CFD) simulations were carried out to optimize the design of the pinching area. We developed a prototype device that can be used to successfully distribute the beads uniformly in eight parallel microwells. The coefficient of variation (CV) of the distributed beads in the microwells was 29.3%.
| Original language | English |
|---|---|
| Pages (from-to) | 281-288 |
| Number of pages | 8 |
| Journal | Sensors and Materials |
| Volume | 20 |
| Issue number | 6 |
| Publication status | Published - 2008 |
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Keywords
- Cellular diagnostics
- High throughput screening
- Microwell array
- Parallel distribution
- Passive distribution
- Self-regulating pinched flow
ASJC Scopus subject areas
- Instrumentation
- Materials Science(all)
Cite this
Parallel and passive distribution to arrayed microwells using self-regulating pinched flow. / Kawai, Kentaro; Kanai, Masaki; Munaka, Tatsuya; Abe, Hirohisa; Murakami, Akira; Shoji, Shuichi.
In: Sensors and Materials, Vol. 20, No. 6, 2008, p. 281-288.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Parallel and passive distribution to arrayed microwells using self-regulating pinched flow
AU - Kawai, Kentaro
AU - Kanai, Masaki
AU - Munaka, Tatsuya
AU - Abe, Hirohisa
AU - Murakami, Akira
AU - Shoji, Shuichi
PY - 2008
Y1 - 2008
N2 - A parallel and passive (without any actuators or electrodes) cell distribution method for a cellular diagnostic microwell array was developed. This method can be used to simultaneously distribute cells evenly to arrayed microwells only by introducing cell suspensions into the inlet. To regulate the dispersion of the number of distributed cells into each microwell, we conceived a novel concept, the self-regulating pinched flow. The self-regulating pinched flow is realized in a functional microchannel, which consists of center micropillar colonnades and side channels. Computational fluid dynamics (CFD) simulations were carried out to optimize the design of the pinching area. We developed a prototype device that can be used to successfully distribute the beads uniformly in eight parallel microwells. The coefficient of variation (CV) of the distributed beads in the microwells was 29.3%.
AB - A parallel and passive (without any actuators or electrodes) cell distribution method for a cellular diagnostic microwell array was developed. This method can be used to simultaneously distribute cells evenly to arrayed microwells only by introducing cell suspensions into the inlet. To regulate the dispersion of the number of distributed cells into each microwell, we conceived a novel concept, the self-regulating pinched flow. The self-regulating pinched flow is realized in a functional microchannel, which consists of center micropillar colonnades and side channels. Computational fluid dynamics (CFD) simulations were carried out to optimize the design of the pinching area. We developed a prototype device that can be used to successfully distribute the beads uniformly in eight parallel microwells. The coefficient of variation (CV) of the distributed beads in the microwells was 29.3%.
KW - Cellular diagnostics
KW - High throughput screening
KW - Microwell array
KW - Parallel distribution
KW - Passive distribution
KW - Self-regulating pinched flow
UR - http://www.scopus.com/inward/record.url?scp=58149231488&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58149231488&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:58149231488
VL - 20
SP - 281
EP - 288
JO - Sensors and Materials
JF - Sensors and Materials
SN - 0914-4935
IS - 6
ER -