Participation of Gs-proteins in the action of relaxin-like gonad-stimulating substance (GSS) for 1-methyladenine production in starfish ovarian follicle cells

Masatoshi Mita, Kazutoshi Yamamoto, Masaru Nakamura, Yuki Takeshige, Miho Watanabe, Yoshitaka Nagahama

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    Gonad-stimulating substance (GSS) in starfish is the only known invertebrate peptide hormone responsible for final gamete maturation, rendering it functionally analogous to gonadotropins in vertebrates. Recently, we purified GSS from radial nerves in the starfish Asterina pectinifera and identified the chemical structure as a heterodimer composed of two different peptides (A- and B-chain) with disulfide cross-linkages. This study examined the hormonal action of GSS on ovarian follicle cells obtained from ovaries in growing (stage IV) and fully grown (stage V) stages, and particularly the mode of signal transduction. The action of GSS on 1-MeAde production by follicle cells in stage V was mediated through the production of cAMP. In contrast, GSS failed to induce 1-MeAde and cAMP production by follicle cells in stage IV. According to competitive experiments using radioiodinated and radioinert GSS, highly specific binding was observed in follicle cells, though their affinities and numbers in stage IV were inferior to those in stage V. Interestingly, Gsα was not detected immunologically in follicle cell membranes of stage IV. Gβ was also faint in stage IV. Although adenylyl cyclase activity in stage V was dose-dependently activated by GSS in the presence of GTP, neither GSS in the presence of GTP nor nonhydrolyzable GTP analogs were effective on the activity in stage IV. These findings strongly suggest that the failure of GSS to produce 1-MeAde is because of a lack of Gs-proteins in follicle cells at stage IV.

    Original languageEnglish
    Pages (from-to)432-437
    Number of pages6
    JournalGeneral and Comparative Endocrinology
    Volume176
    Issue number3
    DOIs
    Publication statusPublished - 2012 May 1

    Fingerprint

    relaxin
    Starfish
    Relaxin
    Ovarian Follicle
    Asteroidea
    ovarian follicles
    Gonads
    gonads
    Proteins
    proteins
    cells
    Guanosine Triphosphate
    Invertebrate Hormones
    Asterina
    Asterina pectinifera
    1-methyladenine
    Radial Nerve
    peptide hormones
    adenylate cyclase
    Peptide Hormones

    Keywords

    • 1-Methyladenine (1-MeAde)
    • Adenylyl cyclase
    • Gonad-stimulating substance (GSS)
    • Gs-proteins
    • Starfish

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Participation of Gs-proteins in the action of relaxin-like gonad-stimulating substance (GSS) for 1-methyladenine production in starfish ovarian follicle cells. / Mita, Masatoshi; Yamamoto, Kazutoshi; Nakamura, Masaru; Takeshige, Yuki; Watanabe, Miho; Nagahama, Yoshitaka.

    In: General and Comparative Endocrinology, Vol. 176, No. 3, 01.05.2012, p. 432-437.

    Research output: Contribution to journalArticle

    Mita, Masatoshi ; Yamamoto, Kazutoshi ; Nakamura, Masaru ; Takeshige, Yuki ; Watanabe, Miho ; Nagahama, Yoshitaka. / Participation of Gs-proteins in the action of relaxin-like gonad-stimulating substance (GSS) for 1-methyladenine production in starfish ovarian follicle cells. In: General and Comparative Endocrinology. 2012 ; Vol. 176, No. 3. pp. 432-437.
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    AU - Watanabe, Miho

    AU - Nagahama, Yoshitaka

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