Passive distributing method for cellular diagnostic microwell array using self-regulating pinched flow

Kentaro Kawai, Masaki Kanai, Tatsuya Munaka, Hirohisa Abe, Akira Murakami, Shuichi Shoji

Research output: Contribution to conferencePaper

Abstract

We developed passive cell distributing method for cellular diagnostic microwell array. This method can distribute cells evenly and simultaneously to a number of microwells only by introducing cell suspensions from the inlet. In order to reduce the dispersion of the number of distributed cells to each microwell, we propose a novel concept of the self-regulating pinched flow. The self-regulating pinched flow is realized in a microchannel, which consists of the center micropillar colonnades and side channels. CFD simulations were carried out to optimize the design of the pinching area. Using a fabricated prototype device having 8 microwells, uniform distribution of the beads was obtained successfully. The coefficient of variation (CV) of the distributed bead in microwells was 29.3%.

Original languageEnglish
Pages500-502
Number of pages3
Publication statusPublished - 2006 Jan 1
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: 2006 Nov 52006 Nov 9

Conference

Conference10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period06/11/506/11/9

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Keywords

  • High throughput screening
  • Microwell array
  • Passive distribution
  • Self-regulating pinched flow

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Kawai, K., Kanai, M., Munaka, T., Abe, H., Murakami, A., & Shoji, S. (2006). Passive distributing method for cellular diagnostic microwell array using self-regulating pinched flow. 500-502. Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.