Passive distributing method for cellular diagnostic microwell array using self-regulating pinched flow

Kentaro Kawai, Masaki Kanai, Tatsuya Munaka, Hirohisa Abe, Akira Murakami, Shuichi Shoji

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    We developed passive cell distributing method for cellular diagnostic microwell array. This method can distribute cells evenly and simultaneously to a number of microwells only by introducing cell suspensions from the inlet. In order to reduce the dispersion of the number of distributed cells to each microwell, we propose a novel concept of the self-regulating pinched flow. The self-regulating pinched flow is realized in a microchannel, which consists of the center micropillar colonnades and side channels. CFD simulations were carried out to optimize the design of the pinching area. Using a fabricated prototype device having 8 microwells, uniform distribution of the beads was obtained successfully. The coefficient of variation (CV) of the distributed bead in microwells was 29.3%.

    Original languageEnglish
    Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    PublisherJapan Academic Association Inc
    Pages500-502
    Number of pages3
    Publication statusPublished - 2006
    Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo
    Duration: 2006 Nov 52006 Nov 9

    Other

    Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
    CityTokyo
    Period06/11/506/11/9

    Fingerprint

    Microchannels
    Suspensions
    Computational fluid dynamics

    Keywords

    • High throughput screening
    • Microwell array
    • Passive distribution
    • Self-regulating pinched flow

    ASJC Scopus subject areas

    • Chemical Engineering (miscellaneous)
    • Bioengineering

    Cite this

    Kawai, K., Kanai, M., Munaka, T., Abe, H., Murakami, A., & Shoji, S. (2006). Passive distributing method for cellular diagnostic microwell array using self-regulating pinched flow. In Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 500-502). Japan Academic Association Inc.

    Passive distributing method for cellular diagnostic microwell array using self-regulating pinched flow. / Kawai, Kentaro; Kanai, Masaki; Munaka, Tatsuya; Abe, Hirohisa; Murakami, Akira; Shoji, Shuichi.

    Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, 2006. p. 500-502.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Kawai, K, Kanai, M, Munaka, T, Abe, H, Murakami, A & Shoji, S 2006, Passive distributing method for cellular diagnostic microwell array using self-regulating pinched flow. in Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, pp. 500-502, 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, 06/11/5.
    Kawai K, Kanai M, Munaka T, Abe H, Murakami A, Shoji S. Passive distributing method for cellular diagnostic microwell array using self-regulating pinched flow. In Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc. 2006. p. 500-502
    Kawai, Kentaro ; Kanai, Masaki ; Munaka, Tatsuya ; Abe, Hirohisa ; Murakami, Akira ; Shoji, Shuichi. / Passive distributing method for cellular diagnostic microwell array using self-regulating pinched flow. Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, 2006. pp. 500-502
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    abstract = "We developed passive cell distributing method for cellular diagnostic microwell array. This method can distribute cells evenly and simultaneously to a number of microwells only by introducing cell suspensions from the inlet. In order to reduce the dispersion of the number of distributed cells to each microwell, we propose a novel concept of the self-regulating pinched flow. The self-regulating pinched flow is realized in a microchannel, which consists of the center micropillar colonnades and side channels. CFD simulations were carried out to optimize the design of the pinching area. Using a fabricated prototype device having 8 microwells, uniform distribution of the beads was obtained successfully. The coefficient of variation (CV) of the distributed bead in microwells was 29.3{\%}.",
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    AU - Kanai, Masaki

    AU - Munaka, Tatsuya

    AU - Abe, Hirohisa

    AU - Murakami, Akira

    AU - Shoji, Shuichi

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