Pathogenesis of acute hepatopancreatic necrosis disease (AHPND) in shrimp

Hung Chiao Lai, Tze Hann Ng, Masahiro Ando, Chung Te Lee, I. Tung Chen, Jie Cheng Chuang, Rapeepat Mavichak, Sheng Hsiung Chang, Mi De Yeh, Yi An Chiang, Haruko Takeyama, Hiro o. Hamaguchi, Chu Fang Lo, Takashi Aoki, Han Ching Wang

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Acute hepatopancreatic necrosis disease (AHPND), also called early mortality syndrome (EMS), is a recently emergent shrimp bacterial disease that has resulted in substantial economic losses since 2009. AHPND is known to be caused by strains of Vibrio parahaemolyticus that contain a unique virulence plasmid, but the pathology of the disease is still unclear. In this study, we show that AHPND-causing strains of V. parahaemolyticus secrete the plasmid–encoded binary toxin PirABvp into the culture medium. We further determined that, after shrimp were challenged with AHPND-causing bacteria, the bacteria initially colonized the stomach, where they started to produce PirABvp toxin. At the same early time point (6 hpi), PirBvp toxin, but not PirAvp toxin, was detected in the hepatopancreas, and the characteristic histopathological signs of AHPND, including sloughing of the epithelial cells of the hepatopancreatic tubules, were also seen. Although some previous studies have found that both components of the binary PirABvp toxin are necessary to induce a toxic effect, our present results are consistent with other studies which have suggested that PirBvp alone may be sufficient to cause cellular damage. At later time points, the bacteria and PirAvp and PirBvp toxins were all detected in the hepatopancreas. We also show that Raman spectroscopy “Whole organism fingerprints” were unable to distinguish between AHPND-causing and non-AHPND causing strains. Lastly, by using minimum inhibitory concentrations, we found that both virulent and non-virulent V. parahaemolyticus strains were resistant to several antibiotics, suggesting that the use of antibiotics in shrimp culture should be more strictly regulated.

Original languageEnglish
Pages (from-to)1006-1014
Number of pages9
JournalFish and Shellfish Immunology
Volume47
Issue number2
DOIs
Publication statusPublished - 2015 Dec 1

Fingerprint

necrosis
shrimp
pathogenesis
toxin
toxins
hepatopancreas
Bacteria
bacteria
antibiotics
bacterium
Raman spectroscopy
shrimp culture
Vibrio parahaemolyticus
bacterial disease
Anti-Bacterial Agents
minimum inhibitory concentration
pathology
virulence
Poisons
plasmid

Keywords

  • Acute hepatopancreatic necrosis disease (AHPND)
  • Early mortality syndrome (EMS)
  • Shrimp
  • Vibrio parahaemolyticus

ASJC Scopus subject areas

  • Environmental Chemistry
  • Aquatic Science

Cite this

Lai, H. C., Ng, T. H., Ando, M., Lee, C. T., Chen, I. T., Chuang, J. C., ... Wang, H. C. (2015). Pathogenesis of acute hepatopancreatic necrosis disease (AHPND) in shrimp. Fish and Shellfish Immunology, 47(2), 1006-1014. https://doi.org/10.1016/j.fsi.2015.11.008

Pathogenesis of acute hepatopancreatic necrosis disease (AHPND) in shrimp. / Lai, Hung Chiao; Ng, Tze Hann; Ando, Masahiro; Lee, Chung Te; Chen, I. Tung; Chuang, Jie Cheng; Mavichak, Rapeepat; Chang, Sheng Hsiung; Yeh, Mi De; Chiang, Yi An; Takeyama, Haruko; Hamaguchi, Hiro o.; Lo, Chu Fang; Aoki, Takashi; Wang, Han Ching.

In: Fish and Shellfish Immunology, Vol. 47, No. 2, 01.12.2015, p. 1006-1014.

Research output: Contribution to journalArticle

Lai, HC, Ng, TH, Ando, M, Lee, CT, Chen, IT, Chuang, JC, Mavichak, R, Chang, SH, Yeh, MD, Chiang, YA, Takeyama, H, Hamaguchi, HO, Lo, CF, Aoki, T & Wang, HC 2015, 'Pathogenesis of acute hepatopancreatic necrosis disease (AHPND) in shrimp', Fish and Shellfish Immunology, vol. 47, no. 2, pp. 1006-1014. https://doi.org/10.1016/j.fsi.2015.11.008
Lai, Hung Chiao ; Ng, Tze Hann ; Ando, Masahiro ; Lee, Chung Te ; Chen, I. Tung ; Chuang, Jie Cheng ; Mavichak, Rapeepat ; Chang, Sheng Hsiung ; Yeh, Mi De ; Chiang, Yi An ; Takeyama, Haruko ; Hamaguchi, Hiro o. ; Lo, Chu Fang ; Aoki, Takashi ; Wang, Han Ching. / Pathogenesis of acute hepatopancreatic necrosis disease (AHPND) in shrimp. In: Fish and Shellfish Immunology. 2015 ; Vol. 47, No. 2. pp. 1006-1014.
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