Pause and rotation of F1-ATPase during catalysis

Yoko Hirono-Hara, Hiroyuki Noji, Masaya Nishiura, Eiro Muneyuki, Kiyotaka Y. Hara, Ryohei Yasuda, Kazuhiko Kinosita, Masasuke Yoshida

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

F1-ATPase is a rotary motor enzyme in which a single ATP molecule drives a 120° rotation of the central γ subunit relative to the surrounding α3β3 ring. Here, we show that the rotation of F1-ATPase spontaneously lapses into long (≅30 s) pauses during steady-state catalysis. The effects of ADP-Mg and mutation on the pauses, as well as kinetic comparison with bulk-phase catalysis, strongly indicate that the paused enzyme corresponds to the inactive state of F1-ATPase previously known as the ADP-Mg inhibited form in which F1-ATPase fails to release ADP-Mg from catalytic sites. The pausing position of the γ subunit deviates from the ATP-waiting position and is most likely the recently found intermediate 90° position.

Original languageEnglish
Pages (from-to)13649-13654
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number24
DOIs
Publication statusPublished - 2001 Nov 20
Externally publishedYes

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Proton-Translocating ATPases
Catalysis
Adenosine Diphosphate
Adenosine Triphosphate
Enzymes
Catalytic Domain
Mutation

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Hirono-Hara, Y., Noji, H., Nishiura, M., Muneyuki, E., Hara, K. Y., Yasuda, R., ... Yoshida, M. (2001). Pause and rotation of F1-ATPase during catalysis. Proceedings of the National Academy of Sciences of the United States of America, 98(24), 13649-13654. https://doi.org/10.1073/pnas.241365698

Pause and rotation of F1-ATPase during catalysis. / Hirono-Hara, Yoko; Noji, Hiroyuki; Nishiura, Masaya; Muneyuki, Eiro; Hara, Kiyotaka Y.; Yasuda, Ryohei; Kinosita, Kazuhiko; Yoshida, Masasuke.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 24, 20.11.2001, p. 13649-13654.

Research output: Contribution to journalArticle

Hirono-Hara, Y, Noji, H, Nishiura, M, Muneyuki, E, Hara, KY, Yasuda, R, Kinosita, K & Yoshida, M 2001, 'Pause and rotation of F1-ATPase during catalysis', Proceedings of the National Academy of Sciences of the United States of America, vol. 98, no. 24, pp. 13649-13654. https://doi.org/10.1073/pnas.241365698
Hirono-Hara, Yoko ; Noji, Hiroyuki ; Nishiura, Masaya ; Muneyuki, Eiro ; Hara, Kiyotaka Y. ; Yasuda, Ryohei ; Kinosita, Kazuhiko ; Yoshida, Masasuke. / Pause and rotation of F1-ATPase during catalysis. In: Proceedings of the National Academy of Sciences of the United States of America. 2001 ; Vol. 98, No. 24. pp. 13649-13654.
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