PCR-DGGE analysis of denitrifying bacteria in a metallurgic wastewater treatment process

N. Noda, S. Yoshie, T. Miyano, Satoshi Tsuneda, A. Hirata, Y. Inamori

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    The wastewater generated from the processes of recovering precious metals from industrial wastes contains high concentrations of acids such as nitric acid and of salts. Biological nitrogen removal from this wastewater was attempted by using a circulating bioreactor system equipped with an anoxic packed bed or an anoxic fluidized bed and an aerobic three-phase fluidized bed. The system was found to effectively remove nitrogen from the diluted wastewater (T-N; 1,000-4,000 mg litre-1). The microbial population structure of activated sludge in an anoxic reactor was analyzed by denaturing gradient gel electrophoresis (DGGE) or PCR-amplified 16S ribosomal DNA (rDNA) fragments. DGGE analysis under different operating conditions demonstrated the presence of some distinguishable bands in the separation pattern, which were most likely derived from many different species constituting the microbial communities. Furthermore, the population diversity varied in accordance with the nitrate-loading rate, water temperature and reactor condition. Some major DGGE bands were excised, reamplified and directly sequenced. It was revealed that the dominant population in the anoxic reactor were affiliated with the β subclass of the class Proteobacteria.

    Original languageEnglish
    Pages (from-to)333-336
    Number of pages4
    JournalWater Science and Technology
    Volume46
    Issue number1-2
    Publication statusPublished - 2002

    Fingerprint

    Electrophoresis
    Wastewater treatment
    electrokinesis
    Bacteria
    Wastewater
    Gels
    gel
    wastewater
    Fluidized beds
    bacterium
    Nitrogen removal
    nitrogen
    Industrial wastes
    precious metal
    Packed beds
    Nitric acid
    Precious metals
    nitric acid
    industrial waste
    Bioreactors

    Keywords

    • 16S rDNA
    • Denitrification
    • Metallurgic wastewater
    • Microbial community
    • PCR-DGGE

    ASJC Scopus subject areas

    • Water Science and Technology

    Cite this

    Noda, N., Yoshie, S., Miyano, T., Tsuneda, S., Hirata, A., & Inamori, Y. (2002). PCR-DGGE analysis of denitrifying bacteria in a metallurgic wastewater treatment process. Water Science and Technology, 46(1-2), 333-336.

    PCR-DGGE analysis of denitrifying bacteria in a metallurgic wastewater treatment process. / Noda, N.; Yoshie, S.; Miyano, T.; Tsuneda, Satoshi; Hirata, A.; Inamori, Y.

    In: Water Science and Technology, Vol. 46, No. 1-2, 2002, p. 333-336.

    Research output: Contribution to journalArticle

    Noda, N, Yoshie, S, Miyano, T, Tsuneda, S, Hirata, A & Inamori, Y 2002, 'PCR-DGGE analysis of denitrifying bacteria in a metallurgic wastewater treatment process', Water Science and Technology, vol. 46, no. 1-2, pp. 333-336.
    Noda, N. ; Yoshie, S. ; Miyano, T. ; Tsuneda, Satoshi ; Hirata, A. ; Inamori, Y. / PCR-DGGE analysis of denitrifying bacteria in a metallurgic wastewater treatment process. In: Water Science and Technology. 2002 ; Vol. 46, No. 1-2. pp. 333-336.
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