PDMS-CYTOP hybrid structure microwell array chip for total internal reflection fluorescence microscopy

A. Nakahara, Y. Shirasaki, Jun Mizuno, Tetsushi Sekiguchi, O. Ohara, Shuichi Shoji

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

A microwell arrry device with a hybrid structure of amorphour perfluoroplymer, CYTOP™, and polydimethylsiloxane (PDMS) which is specialized in total internal refllection fluorescence (TIRF) microscopy was developed. Since the CYTOP layer, whose refractive index is as same as that of water, is sandwiched between PDMS and glass substrate, leakage of incident light is avoided. PDMS layer upon the CYTOP enables high aspect ratio structures and easy integration of microfluidic components. Since direct bonding of CYTOP and PDMS is difficult to achieve, a chemical bonding method using aminosilane coupling reagent and 3-Glycoidoxypropyltriethoxysilane was applied. The bond strength was about 0.74 MPa. 24 × 50 microwell array was fabricated and their optical properties was evaluated. Measured TIRF microscopy results shows no interfere with incident light under the conventionally used conditions.

Original languageEnglish
Title of host publication8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013
Pages743-746
Number of pages4
DOIs
Publication statusPublished - 2013
Externally publishedYes
Event8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013 - Suzhou
Duration: 2013 Apr 72013 Apr 10

Other

Other8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013
CitySuzhou
Period13/4/713/4/10

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Keywords

  • CYTOP
  • microwell array
  • TIRF microscopy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Biotechnology

Cite this

Nakahara, A., Shirasaki, Y., Mizuno, J., Sekiguchi, T., Ohara, O., & Shoji, S. (2013). PDMS-CYTOP hybrid structure microwell array chip for total internal reflection fluorescence microscopy. In 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013 (pp. 743-746). [6559835] https://doi.org/10.1109/NEMS.2013.6559835