PEGylated TiO2 nanoparticles mediated inhibition of cell migration via integrin beta 1

Qingqing Sun, Koki Kanehira, Akiyoshi Taniguchi

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Nanoparticles (NPs) elicit various physiological responses in cellular environment, and the effect of NPs on cell migration is of high interest. In this work, the effects of NPs on cell migration and their possible mechanisms were studied. Here, we showed that after exposure to pegylated titanium dioxide nanoparticles (TiO2-PEG NPs, where PEG stands for the polyethylene glycol), NCI-H292 cells exhibited slower migration than control cells. Furthermore, larger NPs inhibited cell migration much stronger than smaller NPs. Following NP exposure, the cells showed decreased expression of integrin beta 1 and phosphorylated focal adhesion kinase (pFAK), and disrupted F-actin structures. We demonstrated that a possible mechanism involved NP-mediated promotion of the lysosomal degradation of integrin beta 1, thus leading to reduced expression of pFAK and cytoskeletal disruption and inhibited cell migration. Therefore, our results showed that inhibition of NCI-H292 cell migration by NPs is mediated through integrin beta 1, which provides useful information for the application of NPs in cancer therapy and related fields.

Original languageEnglish
Pages (from-to)271-281
Number of pages11
JournalScience and Technology of Advanced Materials
Volume19
Issue number1
DOIs
Publication statusPublished - 2018 Dec 31

Keywords

  • Cell migration
  • TiO-PEG NPs
  • inhibition
  • integrin beta 1
  • pFAK

ASJC Scopus subject areas

  • Materials Science(all)

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