Peptide-based ligand screening system for g protein-coupled receptors using water-in-oil microdroplets

T. Sakurai, R. Iizuka, R. Sekine, Y. Nakamura, Donghyun Yoon, Tetsushi Sekiguchi, J. Ishii, A. Kondo, Shuichi Shoji, T. Funatsu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We present a novel peptide-based ligand screening system for G protein-coupled receptors (GPCRs) in water-in-oil (W/O) microdroplets. This system is an in vitro gene screening system for peptides that activate a target GPCR expressed in yeast. We show that the system is applicable to human GPCRs. This system will be a powerful tool for identifying novel peptide ligands for liganded mammalian GPCRs and orphan mammalian GPCRs.

Original languageEnglish
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages965-967
Number of pages3
ISBN (Print)9780979806476
Publication statusPublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: 2014 Oct 262014 Oct 30

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period14/10/2614/10/30

Keywords

  • Cell-free protein synthesis
  • G protein-coupled receptors
  • Ligand assay
  • Microdroplet
  • Yeast

ASJC Scopus subject areas

  • Control and Systems Engineering

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