Peptide-based ligand screening system for g protein-coupled receptors using water-in-oil microdroplets

T. Sakurai, R. Iizuka, R. Sekine, Y. Nakamura, Donghyun Yoon, Tetsushi Sekiguchi, J. Ishii, A. Kondo, Shuichi Shoji, T. Funatsu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We present a novel peptide-based ligand screening system for G protein-coupled receptors (GPCRs) in water-in-oil (W/O) microdroplets. This system is an in vitro gene screening system for peptides that activate a target GPCR expressed in yeast. We show that the system is applicable to human GPCRs. This system will be a powerful tool for identifying novel peptide ligands for liganded mammalian GPCRs and orphan mammalian GPCRs.

Original languageEnglish
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages965-967
Number of pages3
ISBN (Print)9780979806476
Publication statusPublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: 2014 Oct 262014 Oct 30

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period14/10/2614/10/30

Fingerprint

Peptides
Screening
Ligands
Proteins
Water
Yeast
Genes
Oils

Keywords

  • Cell-free protein synthesis
  • G protein-coupled receptors
  • Ligand assay
  • Microdroplet
  • Yeast

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Sakurai, T., Iizuka, R., Sekine, R., Nakamura, Y., Yoon, D., Sekiguchi, T., ... Funatsu, T. (2014). Peptide-based ligand screening system for g protein-coupled receptors using water-in-oil microdroplets. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 965-967). Chemical and Biological Microsystems Society.

Peptide-based ligand screening system for g protein-coupled receptors using water-in-oil microdroplets. / Sakurai, T.; Iizuka, R.; Sekine, R.; Nakamura, Y.; Yoon, Donghyun; Sekiguchi, Tetsushi; Ishii, J.; Kondo, A.; Shoji, Shuichi; Funatsu, T.

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 965-967.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Sakurai, T, Iizuka, R, Sekine, R, Nakamura, Y, Yoon, D, Sekiguchi, T, Ishii, J, Kondo, A, Shoji, S & Funatsu, T 2014, Peptide-based ligand screening system for g protein-coupled receptors using water-in-oil microdroplets. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, pp. 965-967, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 14/10/26.
Sakurai T, Iizuka R, Sekine R, Nakamura Y, Yoon D, Sekiguchi T et al. Peptide-based ligand screening system for g protein-coupled receptors using water-in-oil microdroplets. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society. 2014. p. 965-967
Sakurai, T. ; Iizuka, R. ; Sekine, R. ; Nakamura, Y. ; Yoon, Donghyun ; Sekiguchi, Tetsushi ; Ishii, J. ; Kondo, A. ; Shoji, Shuichi ; Funatsu, T. / Peptide-based ligand screening system for g protein-coupled receptors using water-in-oil microdroplets. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. pp. 965-967
@inproceedings{6a9717d3d2794809b7850dfb34ea49bb,
title = "Peptide-based ligand screening system for g protein-coupled receptors using water-in-oil microdroplets",
abstract = "We present a novel peptide-based ligand screening system for G protein-coupled receptors (GPCRs) in water-in-oil (W/O) microdroplets. This system is an in vitro gene screening system for peptides that activate a target GPCR expressed in yeast. We show that the system is applicable to human GPCRs. This system will be a powerful tool for identifying novel peptide ligands for liganded mammalian GPCRs and orphan mammalian GPCRs.",
keywords = "Cell-free protein synthesis, G protein-coupled receptors, Ligand assay, Microdroplet, Yeast",
author = "T. Sakurai and R. Iizuka and R. Sekine and Y. Nakamura and Donghyun Yoon and Tetsushi Sekiguchi and J. Ishii and A. Kondo and Shuichi Shoji and T. Funatsu",
year = "2014",
language = "English",
isbn = "9780979806476",
pages = "965--967",
booktitle = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",
publisher = "Chemical and Biological Microsystems Society",

}

TY - GEN

T1 - Peptide-based ligand screening system for g protein-coupled receptors using water-in-oil microdroplets

AU - Sakurai, T.

AU - Iizuka, R.

AU - Sekine, R.

AU - Nakamura, Y.

AU - Yoon, Donghyun

AU - Sekiguchi, Tetsushi

AU - Ishii, J.

AU - Kondo, A.

AU - Shoji, Shuichi

AU - Funatsu, T.

PY - 2014

Y1 - 2014

N2 - We present a novel peptide-based ligand screening system for G protein-coupled receptors (GPCRs) in water-in-oil (W/O) microdroplets. This system is an in vitro gene screening system for peptides that activate a target GPCR expressed in yeast. We show that the system is applicable to human GPCRs. This system will be a powerful tool for identifying novel peptide ligands for liganded mammalian GPCRs and orphan mammalian GPCRs.

AB - We present a novel peptide-based ligand screening system for G protein-coupled receptors (GPCRs) in water-in-oil (W/O) microdroplets. This system is an in vitro gene screening system for peptides that activate a target GPCR expressed in yeast. We show that the system is applicable to human GPCRs. This system will be a powerful tool for identifying novel peptide ligands for liganded mammalian GPCRs and orphan mammalian GPCRs.

KW - Cell-free protein synthesis

KW - G protein-coupled receptors

KW - Ligand assay

KW - Microdroplet

KW - Yeast

UR - http://www.scopus.com/inward/record.url?scp=84941703173&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84941703173&partnerID=8YFLogxK

M3 - Conference contribution

SN - 9780979806476

SP - 965

EP - 967

BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

PB - Chemical and Biological Microsystems Society

ER -