Permeability of anti-fouling PEGylated surfaces probed by fluorescence correlation spectroscopy

Charlisa R. Daniels, Carmen Reznik, Rachel Kilmer, Mary Jane Felipe, Maria Celeste R. Tria, Katerina Kourentzi, Wen Hsiang Chen, Rigoberto C. Advincula, Richard C. Willson, Christy F. Landes

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13 Citations (Scopus)

Abstract

The present work reports on in situ observations of the interaction of organic dye probe molecules and dye-labeled protein with different poly(ethylene glycol) (PEG) architectures (linear, dendron, and bottle brush). Fluorescence correlation spectroscopy (FCS) and single molecule event analysis were used to examine the nature and extent of probe-PEG interactions. The data support a sieve-like model in which size-exclusion principles determine the extent of probe-PEG interactions. Small probes are trapped by more dense PEG architectures and large probes interact more with less dense PEG surfaces. These results, and the tunable pore structure of the PEG dendrons employed in this work, suggest the viability of electrochemically-active materials for tunable surfaces.

Original languageEnglish
Pages (from-to)31-38
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume88
Issue number1
DOIs
Publication statusPublished - 2011 Nov 1
Externally publishedYes

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Keywords

  • Dynamics
  • Fluorescence correlation spectroscopy (FCS)
  • Poly(ethylene glycol) (PEG)
  • Surface interactions
  • Surface modification

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Daniels, C. R., Reznik, C., Kilmer, R., Felipe, M. J., Tria, M. C. R., Kourentzi, K., Chen, W. H., Advincula, R. C., Willson, R. C., & Landes, C. F. (2011). Permeability of anti-fouling PEGylated surfaces probed by fluorescence correlation spectroscopy. Colloids and Surfaces B: Biointerfaces, 88(1), 31-38. https://doi.org/10.1016/j.colsurfb.2011.05.044