Permeability of anti-fouling PEGylated surfaces probed by fluorescence correlation spectroscopy

Charlisa R. Daniels; Carmen Reznik; Rachel Kilmer; Mary Jane Felipe; Maria Celeste R. Tria; Katerina Kourentzi; Wen Hsiang Chen; Rigoberto C. Advincula; Richard C. Willson; Christy F. Landes

doi:10.1016/j.colsurfb.2011.05.044

Permeability of anti-fouling PEGylated surfaces probed by fluorescence correlation spectroscopy

Charlisa R. Daniels, Carmen Reznik, Rachel Kilmer, Mary Jane Felipe, Maria Celeste R. Tria, Katerina Kourentzi, Wen Hsiang Chen, Rigoberto C. Advincula, Richard C. Willson, Christy F. Landes^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

The present work reports on in situ observations of the interaction of organic dye probe molecules and dye-labeled protein with different poly(ethylene glycol) (PEG) architectures (linear, dendron, and bottle brush). Fluorescence correlation spectroscopy (FCS) and single molecule event analysis were used to examine the nature and extent of probe-PEG interactions. The data support a sieve-like model in which size-exclusion principles determine the extent of probe-PEG interactions. Small probes are trapped by more dense PEG architectures and large probes interact more with less dense PEG surfaces. These results, and the tunable pore structure of the PEG dendrons employed in this work, suggest the viability of electrochemically-active materials for tunable surfaces.

Original language	English
Pages (from-to)	31-38
Number of pages	8
Journal	Colloids and Surfaces B: Biointerfaces
Volume	88
Issue number	1
DOIs	https://doi.org/10.1016/j.colsurfb.2011.05.044
Publication status	Published - 2011 Nov 1
Externally published	Yes

Keywords

Dynamics
Fluorescence correlation spectroscopy (FCS)
Poly(ethylene glycol) (PEG)
Surface interactions
Surface modification

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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10.1016/j.colsurfb.2011.05.044

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title = "Permeability of anti-fouling PEGylated surfaces probed by fluorescence correlation spectroscopy",

abstract = "The present work reports on in situ observations of the interaction of organic dye probe molecules and dye-labeled protein with different poly(ethylene glycol) (PEG) architectures (linear, dendron, and bottle brush). Fluorescence correlation spectroscopy (FCS) and single molecule event analysis were used to examine the nature and extent of probe-PEG interactions. The data support a sieve-like model in which size-exclusion principles determine the extent of probe-PEG interactions. Small probes are trapped by more dense PEG architectures and large probes interact more with less dense PEG surfaces. These results, and the tunable pore structure of the PEG dendrons employed in this work, suggest the viability of electrochemically-active materials for tunable surfaces.",

keywords = "Dynamics, Fluorescence correlation spectroscopy (FCS), Poly(ethylene glycol) (PEG), Surface interactions, Surface modification",

author = "Daniels, {Charlisa R.} and Carmen Reznik and Rachel Kilmer and Felipe, {Mary Jane} and Tria, {Maria Celeste R.} and Katerina Kourentzi and Chen, {Wen Hsiang} and Advincula, {Rigoberto C.} and Willson, {Richard C.} and Landes, {Christy F.}",

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doi = "10.1016/j.colsurfb.2011.05.044",

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T1 - Permeability of anti-fouling PEGylated surfaces probed by fluorescence correlation spectroscopy

AU - Daniels, Charlisa R.

AU - Reznik, Carmen

AU - Kilmer, Rachel

AU - Felipe, Mary Jane

AU - Tria, Maria Celeste R.

AU - Kourentzi, Katerina

AU - Chen, Wen Hsiang

AU - Advincula, Rigoberto C.

AU - Willson, Richard C.

AU - Landes, Christy F.

PY - 2011/11/1

Y1 - 2011/11/1

N2 - The present work reports on in situ observations of the interaction of organic dye probe molecules and dye-labeled protein with different poly(ethylene glycol) (PEG) architectures (linear, dendron, and bottle brush). Fluorescence correlation spectroscopy (FCS) and single molecule event analysis were used to examine the nature and extent of probe-PEG interactions. The data support a sieve-like model in which size-exclusion principles determine the extent of probe-PEG interactions. Small probes are trapped by more dense PEG architectures and large probes interact more with less dense PEG surfaces. These results, and the tunable pore structure of the PEG dendrons employed in this work, suggest the viability of electrochemically-active materials for tunable surfaces.

AB - The present work reports on in situ observations of the interaction of organic dye probe molecules and dye-labeled protein with different poly(ethylene glycol) (PEG) architectures (linear, dendron, and bottle brush). Fluorescence correlation spectroscopy (FCS) and single molecule event analysis were used to examine the nature and extent of probe-PEG interactions. The data support a sieve-like model in which size-exclusion principles determine the extent of probe-PEG interactions. Small probes are trapped by more dense PEG architectures and large probes interact more with less dense PEG surfaces. These results, and the tunable pore structure of the PEG dendrons employed in this work, suggest the viability of electrochemically-active materials for tunable surfaces.

KW - Dynamics

KW - Fluorescence correlation spectroscopy (FCS)

KW - Poly(ethylene glycol) (PEG)

KW - Surface interactions

KW - Surface modification

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JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

IS - 1

ER -

Permeability of anti-fouling PEGylated surfaces probed by fluorescence correlation spectroscopy

Abstract

Keywords

ASJC Scopus subject areas

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