Persistent colonization of non-lymphoid tissue-resident macrophages by Stenotrophomonas maltophilia

Ichiro Takahashi, Koji Hosomi, Takahiro Nagatake, Hirokazu Toubou, Daiki Yamamoto, Ikue Hayashi, Yosuke Kurashima, Shintaro Sato, Naoko Shibata, Yoshiyuki Goto, Fumito Maruyama, Ichiro Nakagawa, Asaomi Kuwae, Akio Abe, Jun Kunisawa*, Hiroshi Kiyono

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Accumulating evidence has revealed that lymphoid tissue-resident commensal bacteria (e.g. Alcaligenes spp.) survive within dendritic cells. We extended our previous study by investigating microbes that persistently colonize colonic macrophages. 16S rRNA-based metagenome analysis using DNA purified from murine colonic macrophages revealed the presence of Stenotrophomonas maltophilia. The in situ intracellular colonization by S. maltophilia was recapitulated in vitro by using bone marrow-derived macrophages (BMDMs). Co-culture of BMDMs with clinically isolated S. maltophilia led to increased mitochondrial respiration and robust IL-10 production. We further identified a 25-kDa protein encoded by the gene assigned as smlt2713 (recently renamed as SMLT-RS12935) and secreted by S. maltophilia as the factor responsible for enhanced IL-10 production by BMDMs. IL-10 production is critical for maintenance of the symbiotic condition, because intracellular colonization by S. maltophilia was impaired in IL-10-deficient BMDMs, and smlt2713-deficient S. maltophilia failed to persistently colonize IL-10-competent BMDMs. These findings indicate a novel commensal network between colonic macrophages and S. maltophilia that is mediated by IL-10 and smlt2713.

Original languageEnglish
Pages (from-to)133-141
Number of pages9
JournalInternational Immunology
Volume32
Issue number2
DOIs
Publication statusPublished - 2019 Nov 12
Externally publishedYes

Keywords

  • IL-10
  • colitis
  • gut-resident macrophage
  • intracellular commensal bacteria
  • symbiotic factor smlt2713

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint

Dive into the research topics of 'Persistent colonization of non-lymphoid tissue-resident macrophages by Stenotrophomonas maltophilia'. Together they form a unique fingerprint.

Cite this