Pestynol, an Antifungal Compound Discovered Using a Saccharomyces cerevisiae 12geneΔ0HSR-iERG6-Based Assay

Katsuyuki Sakai, Tomoyasu Hirose, Masato Iwatsuki, Takumi Chinen, Toru Kimura, Takuya Suga, Kenichi Nonaka, Takuji Nakashima, Toshiaki Sunazuka, Takeo Usui, Yukihiro Asami, Satoshi Omura, Kazuro Shiomi

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The multidrug-sensitive budding yeast, Saccharomyces cerevisiae 12geneΔ0HSR-iERG6, is very useful in antifungal screens. A novel compound, named pestynol (1), was discovered from a culture of the fungus Pestalotiopsis humus FKI-7473 using the multidrug-sensitive yeast. The structure of 1 was elucidated by NMR studies and modified Mosher's method as (1R,2R,3R,4R)-(E)-5-(7,11-dimethyl-3-methylenedodeca-6,10-dien-1-yn-1-yl)cyclohex-5-ene-1,2,3,4-tetraol. Compound 1 showed antimicrobial activity against the Gram-positive bacteria, Klebsiella pneumoniae, and S. cerevisiae 12geneΔ0HSR-iERG6 and Mucor racemosus, but displayed only weak cytotoxicity against various human cancer cell lines. Compound 1 displayed antifungal activities against S. cerevisiae 12geneΔ0HSR-iERG6 and Mucor racemosus at 10 μg/disc.

Original languageEnglish
Pages (from-to)1604-1609
Number of pages6
JournalJournal of Natural Products
Volume81
Issue number7
DOIs
Publication statusPublished - 2018 Jul 27
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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