Pestynol, an Antifungal Compound Discovered Using a Saccharomyces cerevisiae 12geneΔ0HSR-iERG6-Based Assay

Katsuyuki Sakai; Tomoyasu Hirose; Masato Iwatsuki; Takumi Chinen; Toru Kimura; Takuya Suga; Kenichi Nonaka; Takuji Nakashima; Toshiaki Sunazuka; Takeo Usui; Yukihiro Asami; Satoshi Omura; Kazuro Shiomi

doi:10.1021/acs.jnatprod.8b00200

Pestynol, an Antifungal Compound Discovered Using a Saccharomyces cerevisiae 12geneΔ0HSR-iERG6-Based Assay

Katsuyuki Sakai, Tomoyasu Hirose, Masato Iwatsuki, Takumi Chinen, Toru Kimura, Takuya Suga, Kenichi Nonaka, Takuji Nakashima, Toshiaki Sunazuka, Takeo Usui, Yukihiro Asami, Satoshi Omura^*, Kazuro Shiomi

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

The multidrug-sensitive budding yeast, Saccharomyces cerevisiae 12geneΔ0HSR-iERG6, is very useful in antifungal screens. A novel compound, named pestynol (1), was discovered from a culture of the fungus Pestalotiopsis humus FKI-7473 using the multidrug-sensitive yeast. The structure of 1 was elucidated by NMR studies and modified Mosher's method as (1R,2R,3R,4R)-(E)-5-(7,11-dimethyl-3-methylenedodeca-6,10-dien-1-yn-1-yl)cyclohex-5-ene-1,2,3,4-tetraol. Compound 1 showed antimicrobial activity against the Gram-positive bacteria, Klebsiella pneumoniae, and S. cerevisiae 12geneΔ0HSR-iERG6 and Mucor racemosus, but displayed only weak cytotoxicity against various human cancer cell lines. Compound 1 displayed antifungal activities against S. cerevisiae 12geneΔ0HSR-iERG6 and Mucor racemosus at 10 μg/disc.

Original language	English
Pages (from-to)	1604-1609
Number of pages	6
Journal	Journal of Natural Products
Volume	81
Issue number	7
DOIs	https://doi.org/10.1021/acs.jnatprod.8b00200
Publication status	Published - 2018 Jul 27
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Molecular Medicine
Pharmacology
Pharmaceutical Science
Drug Discovery
Complementary and alternative medicine
Organic Chemistry

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/acs.jnatprod.8b00200

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title = "Pestynol, an Antifungal Compound Discovered Using a Saccharomyces cerevisiae 12geneΔ0HSR-iERG6-Based Assay",

abstract = "The multidrug-sensitive budding yeast, Saccharomyces cerevisiae 12geneΔ0HSR-iERG6, is very useful in antifungal screens. A novel compound, named pestynol (1), was discovered from a culture of the fungus Pestalotiopsis humus FKI-7473 using the multidrug-sensitive yeast. The structure of 1 was elucidated by NMR studies and modified Mosher's method as (1R,2R,3R,4R)-(E)-5-(7,11-dimethyl-3-methylenedodeca-6,10-dien-1-yn-1-yl)cyclohex-5-ene-1,2,3,4-tetraol. Compound 1 showed antimicrobial activity against the Gram-positive bacteria, Klebsiella pneumoniae, and S. cerevisiae 12geneΔ0HSR-iERG6 and Mucor racemosus, but displayed only weak cytotoxicity against various human cancer cell lines. Compound 1 displayed antifungal activities against S. cerevisiae 12geneΔ0HSR-iERG6 and Mucor racemosus at 10 μg/disc.",

author = "Katsuyuki Sakai and Tomoyasu Hirose and Masato Iwatsuki and Takumi Chinen and Toru Kimura and Takuya Suga and Kenichi Nonaka and Takuji Nakashima and Toshiaki Sunazuka and Takeo Usui and Yukihiro Asami and Satoshi Omura and Kazuro Shiomi",

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doi = "10.1021/acs.jnatprod.8b00200",

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AU - Sakai, Katsuyuki

AU - Hirose, Tomoyasu

AU - Iwatsuki, Masato

AU - Chinen, Takumi

AU - Kimura, Toru

AU - Suga, Takuya

AU - Nonaka, Kenichi

AU - Nakashima, Takuji

AU - Sunazuka, Toshiaki

AU - Usui, Takeo

AU - Asami, Yukihiro

AU - Omura, Satoshi

AU - Shiomi, Kazuro

PY - 2018/7/27

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AB - The multidrug-sensitive budding yeast, Saccharomyces cerevisiae 12geneΔ0HSR-iERG6, is very useful in antifungal screens. A novel compound, named pestynol (1), was discovered from a culture of the fungus Pestalotiopsis humus FKI-7473 using the multidrug-sensitive yeast. The structure of 1 was elucidated by NMR studies and modified Mosher's method as (1R,2R,3R,4R)-(E)-5-(7,11-dimethyl-3-methylenedodeca-6,10-dien-1-yn-1-yl)cyclohex-5-ene-1,2,3,4-tetraol. Compound 1 showed antimicrobial activity against the Gram-positive bacteria, Klebsiella pneumoniae, and S. cerevisiae 12geneΔ0HSR-iERG6 and Mucor racemosus, but displayed only weak cytotoxicity against various human cancer cell lines. Compound 1 displayed antifungal activities against S. cerevisiae 12geneΔ0HSR-iERG6 and Mucor racemosus at 10 μg/disc.

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Pestynol, an Antifungal Compound Discovered Using a Saccharomyces cerevisiae 12geneΔ0HSR-iERG6-Based Assay

Abstract

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