PGC7/Stella protects against DNA demethylation in early embryogenesis

Toshinobu Nakamura, Yoshikazu Arai, Hiroki Umehara, Masaaki Masuhara, Tohru Kimura, Hisaaki Taniguchi, Toshihiro Sekimoto, Masahito Ikawa, Yoshihiro Yoneda, Masaru Okabe, Satoshi Tanaka, Kunio Shiota, Toru Nakano

Research output: Contribution to journalArticle

387 Citations (Scopus)

Abstract

DNA methylation is an important means of epigenetic gene regulation and must be carefully controlled as a prerequisite for normal early embryogenesis. Although global demethylation occurs soon after fertilization, it is not evenly distributed throughout the genome. Genomic imprinting and epigenetic asymmetry between parental genomes, that is, delayed demethylation of the maternal genome after fertilization, are clear examples of the functional importance of DNA methylation. Here, we show that PGC7/Stella, a maternal factor essential for early development, protects the DNA methylation state of several imprinted loci and epigenetic asymmetry. After determining that PGC7/Stella binds to Ran binding protein 5 (RanBP5; a nuclear transport shuttle protein), mutant versions of the two proteins were used to examine exactly when and where PGC7/Stella functions within the cell. It is likely that PGC7/Stella protects the maternal genome from demethylation only after localizing to the nucleus, where it maintains the methylation of several imprinted genes. These results demonstrate that PGC7/Stella is indispensable for the maintenance of methylation involved in epigenetic reprogramming after fertilization.

Original languageEnglish
Pages (from-to)64-71
Number of pages8
JournalNature Cell Biology
Volume9
Issue number1
DOIs
Publication statusPublished - 2007 Jan
Externally publishedYes

Fingerprint

Epigenomics
Embryonic Development
DNA Methylation
Fertilization
Genome
DNA
Mothers
Methylation
Carrier Proteins
ran GTP-Binding Protein
Genomic Imprinting
Cell Nucleus Active Transport
Nuclear Proteins
Genes
Maintenance
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Nakamura, T., Arai, Y., Umehara, H., Masuhara, M., Kimura, T., Taniguchi, H., ... Nakano, T. (2007). PGC7/Stella protects against DNA demethylation in early embryogenesis. Nature Cell Biology, 9(1), 64-71. https://doi.org/10.1038/ncb1519

PGC7/Stella protects against DNA demethylation in early embryogenesis. / Nakamura, Toshinobu; Arai, Yoshikazu; Umehara, Hiroki; Masuhara, Masaaki; Kimura, Tohru; Taniguchi, Hisaaki; Sekimoto, Toshihiro; Ikawa, Masahito; Yoneda, Yoshihiro; Okabe, Masaru; Tanaka, Satoshi; Shiota, Kunio; Nakano, Toru.

In: Nature Cell Biology, Vol. 9, No. 1, 01.2007, p. 64-71.

Research output: Contribution to journalArticle

Nakamura, T, Arai, Y, Umehara, H, Masuhara, M, Kimura, T, Taniguchi, H, Sekimoto, T, Ikawa, M, Yoneda, Y, Okabe, M, Tanaka, S, Shiota, K & Nakano, T 2007, 'PGC7/Stella protects against DNA demethylation in early embryogenesis', Nature Cell Biology, vol. 9, no. 1, pp. 64-71. https://doi.org/10.1038/ncb1519
Nakamura T, Arai Y, Umehara H, Masuhara M, Kimura T, Taniguchi H et al. PGC7/Stella protects against DNA demethylation in early embryogenesis. Nature Cell Biology. 2007 Jan;9(1):64-71. https://doi.org/10.1038/ncb1519
Nakamura, Toshinobu ; Arai, Yoshikazu ; Umehara, Hiroki ; Masuhara, Masaaki ; Kimura, Tohru ; Taniguchi, Hisaaki ; Sekimoto, Toshihiro ; Ikawa, Masahito ; Yoneda, Yoshihiro ; Okabe, Masaru ; Tanaka, Satoshi ; Shiota, Kunio ; Nakano, Toru. / PGC7/Stella protects against DNA demethylation in early embryogenesis. In: Nature Cell Biology. 2007 ; Vol. 9, No. 1. pp. 64-71.
@article{71e52b11137d4d968768950c0ea13651,
title = "PGC7/Stella protects against DNA demethylation in early embryogenesis",
abstract = "DNA methylation is an important means of epigenetic gene regulation and must be carefully controlled as a prerequisite for normal early embryogenesis. Although global demethylation occurs soon after fertilization, it is not evenly distributed throughout the genome. Genomic imprinting and epigenetic asymmetry between parental genomes, that is, delayed demethylation of the maternal genome after fertilization, are clear examples of the functional importance of DNA methylation. Here, we show that PGC7/Stella, a maternal factor essential for early development, protects the DNA methylation state of several imprinted loci and epigenetic asymmetry. After determining that PGC7/Stella binds to Ran binding protein 5 (RanBP5; a nuclear transport shuttle protein), mutant versions of the two proteins were used to examine exactly when and where PGC7/Stella functions within the cell. It is likely that PGC7/Stella protects the maternal genome from demethylation only after localizing to the nucleus, where it maintains the methylation of several imprinted genes. These results demonstrate that PGC7/Stella is indispensable for the maintenance of methylation involved in epigenetic reprogramming after fertilization.",
author = "Toshinobu Nakamura and Yoshikazu Arai and Hiroki Umehara and Masaaki Masuhara and Tohru Kimura and Hisaaki Taniguchi and Toshihiro Sekimoto and Masahito Ikawa and Yoshihiro Yoneda and Masaru Okabe and Satoshi Tanaka and Kunio Shiota and Toru Nakano",
year = "2007",
month = "1",
doi = "10.1038/ncb1519",
language = "English",
volume = "9",
pages = "64--71",
journal = "Nature Cell Biology",
issn = "1465-7392",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - PGC7/Stella protects against DNA demethylation in early embryogenesis

AU - Nakamura, Toshinobu

AU - Arai, Yoshikazu

AU - Umehara, Hiroki

AU - Masuhara, Masaaki

AU - Kimura, Tohru

AU - Taniguchi, Hisaaki

AU - Sekimoto, Toshihiro

AU - Ikawa, Masahito

AU - Yoneda, Yoshihiro

AU - Okabe, Masaru

AU - Tanaka, Satoshi

AU - Shiota, Kunio

AU - Nakano, Toru

PY - 2007/1

Y1 - 2007/1

N2 - DNA methylation is an important means of epigenetic gene regulation and must be carefully controlled as a prerequisite for normal early embryogenesis. Although global demethylation occurs soon after fertilization, it is not evenly distributed throughout the genome. Genomic imprinting and epigenetic asymmetry between parental genomes, that is, delayed demethylation of the maternal genome after fertilization, are clear examples of the functional importance of DNA methylation. Here, we show that PGC7/Stella, a maternal factor essential for early development, protects the DNA methylation state of several imprinted loci and epigenetic asymmetry. After determining that PGC7/Stella binds to Ran binding protein 5 (RanBP5; a nuclear transport shuttle protein), mutant versions of the two proteins were used to examine exactly when and where PGC7/Stella functions within the cell. It is likely that PGC7/Stella protects the maternal genome from demethylation only after localizing to the nucleus, where it maintains the methylation of several imprinted genes. These results demonstrate that PGC7/Stella is indispensable for the maintenance of methylation involved in epigenetic reprogramming after fertilization.

AB - DNA methylation is an important means of epigenetic gene regulation and must be carefully controlled as a prerequisite for normal early embryogenesis. Although global demethylation occurs soon after fertilization, it is not evenly distributed throughout the genome. Genomic imprinting and epigenetic asymmetry between parental genomes, that is, delayed demethylation of the maternal genome after fertilization, are clear examples of the functional importance of DNA methylation. Here, we show that PGC7/Stella, a maternal factor essential for early development, protects the DNA methylation state of several imprinted loci and epigenetic asymmetry. After determining that PGC7/Stella binds to Ran binding protein 5 (RanBP5; a nuclear transport shuttle protein), mutant versions of the two proteins were used to examine exactly when and where PGC7/Stella functions within the cell. It is likely that PGC7/Stella protects the maternal genome from demethylation only after localizing to the nucleus, where it maintains the methylation of several imprinted genes. These results demonstrate that PGC7/Stella is indispensable for the maintenance of methylation involved in epigenetic reprogramming after fertilization.

UR - http://www.scopus.com/inward/record.url?scp=33845885282&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845885282&partnerID=8YFLogxK

U2 - 10.1038/ncb1519

DO - 10.1038/ncb1519

M3 - Article

C2 - 17143267

AN - SCOPUS:33845885282

VL - 9

SP - 64

EP - 71

JO - Nature Cell Biology

JF - Nature Cell Biology

SN - 1465-7392

IS - 1

ER -