Phagocytosis of bacterial magnetite by leucocytes

Tadashi Matsunaga, Kohji Hashimoto, Noriyuki Nakamura, Kayoko Nakamura, Shozo Hashimoto

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Magnetotactic bacteria were introduced into granulocytes and monocytes by phagocytosis. The number of phagocytes containing bacterial magnetites (magneto-sensitive cells) became constant after 1.5 h incubation, and viable phagocytes contained about 20-40 cells of magnetotactic bacteria. Granulocytes and monocytes containing bacterial magnetites were separated by magnet a Samarium-cobalt from lymphocytes. After separation, 89% of lymphocytes were recovered and 95% of the cells were viable. The contamination of phagocytes in the recovered lymphocytes was below 0.8%. Magneto-sensitive granulocytes and monocytes were removed by applying a magnetic field. The nitro-blue tetrazolium-reducing, chemotactic and phagocytic abilities of phagocytes ingesting magnetotactic bacteria were 84%, 88% and 87% respectively after 1 h incubation.

Original languageEnglish
Pages (from-to)401-405
Number of pages5
JournalApplied Microbiology and Biotechnology
Volume31
Issue number4
DOIs
Publication statusPublished - 1989 Sep
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology
  • Bioengineering
  • Biotechnology

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  • Cite this

    Matsunaga, T., Hashimoto, K., Nakamura, N., Nakamura, K., & Hashimoto, S. (1989). Phagocytosis of bacterial magnetite by leucocytes. Applied Microbiology and Biotechnology, 31(4), 401-405. https://doi.org/10.1007/BF00257612