Phase advances of circadian rhythms in somatostatin depleted rats: effects of cysteamine on rhythms of locomotor activity and electrical discharge of the suprachiasmatic nucleus

C. Fukuhara, S. I T Inouye, K. Aoki, T. Hamada, Shigenobu Shibata, S. Watanabe

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Somatostatin is synthesized in the suprachiasmatic nucleus (SCN), a circadian pacemaker in mammals. To explore the functional significance of somatostatin in the circadian system, we examined rhythms of rat locomotor activity and electrical firing rate of SCN neurons in the brain slice after temporal depletion of somatostatin levels in the SCN. Intraperitoneal administration of cysteamine (200 mg/kg), a somatostatin depletor, significantly reduced somatostatin level in the in vivo SCN 5 min after injection and kept low level as long as 3 to 4 days. This administration, on the other hand, induced significant phase advances of about 51 min in the subsequent free-running rhythm of locomotor activity of the rat. A marked phase advance in the circadian rhythm of firing rate in the SCN was also observed after administration of cysteamine in coronal hypothalamic slices. These persistent phase shifts after administration of a somatostatin depletor may suggest that the change of somatostatin level in the SCN have a feedback influence on the circadian pacemaker.

Original languageEnglish
Pages (from-to)677-685
Number of pages9
JournalJournal of Comparative Physiology A
Volume175
Issue number6
DOIs
Publication statusPublished - 1994 Dec
Externally publishedYes

Fingerprint

cysteamine
Cysteamine
circadian rhythm
Suprachiasmatic Nucleus
somatostatin
Locomotion
Circadian Rhythm
Somatostatin
locomotion
rats
brain
mammal
intraperitoneal injection
effect
Running
Mammals
neurons
mammals
injection
Neurons

Keywords

  • Brain slice
  • Circadian rhythm
  • Cysteamine
  • Somatostatin
  • Suprachiasmatic nucleus

ASJC Scopus subject areas

  • Behavioral Neuroscience
  • Neuroscience(all)
  • Physiology (medical)
  • Physiology
  • Animal Science and Zoology

Cite this

Phase advances of circadian rhythms in somatostatin depleted rats : effects of cysteamine on rhythms of locomotor activity and electrical discharge of the suprachiasmatic nucleus. / Fukuhara, C.; Inouye, S. I T; Aoki, K.; Hamada, T.; Shibata, Shigenobu; Watanabe, S.

In: Journal of Comparative Physiology A, Vol. 175, No. 6, 12.1994, p. 677-685.

Research output: Contribution to journalArticle

@article{bad3ecea4f254b809af27b32cc1d36ca,
title = "Phase advances of circadian rhythms in somatostatin depleted rats: effects of cysteamine on rhythms of locomotor activity and electrical discharge of the suprachiasmatic nucleus",
abstract = "Somatostatin is synthesized in the suprachiasmatic nucleus (SCN), a circadian pacemaker in mammals. To explore the functional significance of somatostatin in the circadian system, we examined rhythms of rat locomotor activity and electrical firing rate of SCN neurons in the brain slice after temporal depletion of somatostatin levels in the SCN. Intraperitoneal administration of cysteamine (200 mg/kg), a somatostatin depletor, significantly reduced somatostatin level in the in vivo SCN 5 min after injection and kept low level as long as 3 to 4 days. This administration, on the other hand, induced significant phase advances of about 51 min in the subsequent free-running rhythm of locomotor activity of the rat. A marked phase advance in the circadian rhythm of firing rate in the SCN was also observed after administration of cysteamine in coronal hypothalamic slices. These persistent phase shifts after administration of a somatostatin depletor may suggest that the change of somatostatin level in the SCN have a feedback influence on the circadian pacemaker.",
keywords = "Brain slice, Circadian rhythm, Cysteamine, Somatostatin, Suprachiasmatic nucleus",
author = "C. Fukuhara and Inouye, {S. I T} and K. Aoki and T. Hamada and Shigenobu Shibata and S. Watanabe",
year = "1994",
month = "12",
doi = "10.1007/BF00191840",
language = "English",
volume = "175",
pages = "677--685",
journal = "Journal of Comparative Physiology A: Neuroethology, Sensory, Neural, and Behavioral Physiology",
issn = "0340-7594",
publisher = "Springer Verlag",
number = "6",

}

TY - JOUR

T1 - Phase advances of circadian rhythms in somatostatin depleted rats

T2 - effects of cysteamine on rhythms of locomotor activity and electrical discharge of the suprachiasmatic nucleus

AU - Fukuhara, C.

AU - Inouye, S. I T

AU - Aoki, K.

AU - Hamada, T.

AU - Shibata, Shigenobu

AU - Watanabe, S.

PY - 1994/12

Y1 - 1994/12

N2 - Somatostatin is synthesized in the suprachiasmatic nucleus (SCN), a circadian pacemaker in mammals. To explore the functional significance of somatostatin in the circadian system, we examined rhythms of rat locomotor activity and electrical firing rate of SCN neurons in the brain slice after temporal depletion of somatostatin levels in the SCN. Intraperitoneal administration of cysteamine (200 mg/kg), a somatostatin depletor, significantly reduced somatostatin level in the in vivo SCN 5 min after injection and kept low level as long as 3 to 4 days. This administration, on the other hand, induced significant phase advances of about 51 min in the subsequent free-running rhythm of locomotor activity of the rat. A marked phase advance in the circadian rhythm of firing rate in the SCN was also observed after administration of cysteamine in coronal hypothalamic slices. These persistent phase shifts after administration of a somatostatin depletor may suggest that the change of somatostatin level in the SCN have a feedback influence on the circadian pacemaker.

AB - Somatostatin is synthesized in the suprachiasmatic nucleus (SCN), a circadian pacemaker in mammals. To explore the functional significance of somatostatin in the circadian system, we examined rhythms of rat locomotor activity and electrical firing rate of SCN neurons in the brain slice after temporal depletion of somatostatin levels in the SCN. Intraperitoneal administration of cysteamine (200 mg/kg), a somatostatin depletor, significantly reduced somatostatin level in the in vivo SCN 5 min after injection and kept low level as long as 3 to 4 days. This administration, on the other hand, induced significant phase advances of about 51 min in the subsequent free-running rhythm of locomotor activity of the rat. A marked phase advance in the circadian rhythm of firing rate in the SCN was also observed after administration of cysteamine in coronal hypothalamic slices. These persistent phase shifts after administration of a somatostatin depletor may suggest that the change of somatostatin level in the SCN have a feedback influence on the circadian pacemaker.

KW - Brain slice

KW - Circadian rhythm

KW - Cysteamine

KW - Somatostatin

KW - Suprachiasmatic nucleus

UR - http://www.scopus.com/inward/record.url?scp=0028630513&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028630513&partnerID=8YFLogxK

U2 - 10.1007/BF00191840

DO - 10.1007/BF00191840

M3 - Article

C2 - 7807413

AN - SCOPUS:0028630513

VL - 175

SP - 677

EP - 685

JO - Journal of Comparative Physiology A: Neuroethology, Sensory, Neural, and Behavioral Physiology

JF - Journal of Comparative Physiology A: Neuroethology, Sensory, Neural, and Behavioral Physiology

SN - 0340-7594

IS - 6

ER -