Phase separation of polymerized mixed liposomes. Analysis of release behavior of entrapped molecules with skeletonization

Shinji Takeoka, Hiromi Sakai, Hiroyuki Ohno, Eishun Tsuchida

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    The mixed liposomes, composed of a polymerizable lipid, 1,2-bis(2,4-octadecadienoyl)-sn-glycero-3-phosphorylcholine (DODPC), and nonpolymerizable membrane constituents, DPPC, cholesterol, and sodium didodecyl phosphate, were prepared by an extrusion method. After polymerization, nonpolymerizable constituents were removed to obtain the polymerized framework of the liposome (the skeletonized liposome). The release of small molecules from the skeletonized liposomes through resulting holes was analyzed. A 5(6)-carboxyfluorescein (CF) and saccharides with various molecular weights were applied as release molecules. The molecular weight of dextran, whose retention ratio in the skeletonized liposomes is 50%, relates to the apparent size of the holes, i.e., the apparent domain size of the phase separation of a polymerized mixed liposome. The size of the holes increases with an increase in the mole fraction of nonpolymerizable lipids. This also depends on the polymerization temperature and the structure of nonpolymerizable lipids.

    Original languageEnglish
    Pages (from-to)1279-1283
    Number of pages5
    JournalMacromolecules
    Volume24
    Issue number6
    Publication statusPublished - 1991 Jan 1

    Fingerprint

    Liposomes
    Phase separation
    Molecules
    Lipids
    Molecular weight
    Polymerization
    Phosphorylcholine
    Dextran
    Cholesterol
    Dextrans
    Extrusion
    Phosphates
    Sodium
    Membranes

    ASJC Scopus subject areas

    • Materials Chemistry

    Cite this

    Phase separation of polymerized mixed liposomes. Analysis of release behavior of entrapped molecules with skeletonization. / Takeoka, Shinji; Sakai, Hiromi; Ohno, Hiroyuki; Tsuchida, Eishun.

    In: Macromolecules, Vol. 24, No. 6, 01.01.1991, p. 1279-1283.

    Research output: Contribution to journalArticle

    Takeoka, Shinji ; Sakai, Hiromi ; Ohno, Hiroyuki ; Tsuchida, Eishun. / Phase separation of polymerized mixed liposomes. Analysis of release behavior of entrapped molecules with skeletonization. In: Macromolecules. 1991 ; Vol. 24, No. 6. pp. 1279-1283.
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