Phenotypes of gene disruptants in relation to a putative mitochondrial malate-citrate shuttle protein in citric acid-producing Aspergillus niger

Kohtaro Kirimura, Keiichi Kobayashi, Yuka Ueda, Takasumi Hattori

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The mitochondrial citrate transport protein (CTP) functions as a malate-citrate shuttle catalyzing the exchange of citrate plus a proton for malate between mitochondria and cytosol across the inner mitochondrial membrane in higher eukaryotic organisms. In this study, for functional analysis, we cloned the gene encoding putative CTP (ctpA) of citric acid-producing Aspergillus niger WU-2223L. The gene ctpA encodes a polypeptide consisting 296 amino acids conserved active residues required for citrate transport function. Only in early-log phase, the ctpA disruptant DCTPA-1 showed growth delay, and the amount of citric acid produced by strain DCTPA-1 was smaller than that by parental strain WU-2223L. These results indicate that the CTPA affects growth and thereby citric acid metabolism of A. niger changes, especially in early-log phase, but not citric acid-producing period. This is the first report showing that disruption of ctpA causes changes of phenotypes in relation to citric acid production in A. niger.

Original languageEnglish
Pages (from-to)1737-1746
Number of pages10
JournalBioscience, Biotechnology and Biochemistry
Volume80
Issue number9
DOIs
Publication statusPublished - 2016

Keywords

  • Aspergillus niger
  • Citrate transport protein
  • Citric acid production
  • Disruption of ctpA
  • Malate-citrate shuttle

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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