Phenotypes of gene disruptants in relation to a putative mitochondrial malate-citrate shuttle protein in citric acid-producing Aspergillus niger

Kotaro Kirimura, Keiichi Kobayashi, Yuka Ueda, Takasumi Hattori

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    The mitochondrial citrate transport protein (CTP) functions as a malate-citrate shuttle catalyzing the exchange of citrate plus a proton for malate between mitochondria and cytosol across the inner mitochondrial membrane in higher eukaryotic organisms. In this study, for functional analysis, we cloned the gene encoding putative CTP (ctpA) of citric acid-producing Aspergillus niger WU-2223L. The gene ctpA encodes a polypeptide consisting 296 amino acids conserved active residues required for citrate transport function. Only in early-log phase, the ctpA disruptant DCTPA-1 showed growth delay, and the amount of citric acid produced by strain DCTPA-1 was smaller than that by parental strain WU-2223L. These results indicate that the CTPA affects growth and thereby citric acid metabolism of A. niger changes, especially in early-log phase, but not citric acid-producing period. This is the first report showing that disruption of ctpA causes changes of phenotypes in relation to citric acid production in A. niger.

    Original languageEnglish
    Pages (from-to)1737-1746
    Number of pages10
    JournalBioscience, Biotechnology and Biochemistry
    Volume80
    Issue number9
    DOIs
    Publication statusPublished - 2016

    Fingerprint

    Aspergillus niger
    Aspergillus
    Citric Acid
    Genes
    Phenotype
    Proteins
    malic acid
    Functional analysis
    Mitochondria
    Gene encoding
    Mitochondrial Membranes
    Growth
    Metabolism
    Cytosol
    Protons
    Carrier Proteins
    Membranes
    Amino Acids
    Peptides

    Keywords

    • Aspergillus niger
    • Citrate transport protein
    • Citric acid production
    • Disruption of ctpA
    • Malate-citrate shuttle

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Molecular Biology
    • Organic Chemistry

    Cite this

    Phenotypes of gene disruptants in relation to a putative mitochondrial malate-citrate shuttle protein in citric acid-producing Aspergillus niger. / Kirimura, Kotaro; Kobayashi, Keiichi; Ueda, Yuka; Hattori, Takasumi.

    In: Bioscience, Biotechnology and Biochemistry, Vol. 80, No. 9, 2016, p. 1737-1746.

    Research output: Contribution to journalArticle

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    AU - Hattori, Takasumi

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