Phenylalanine methyl ester production from phenylpyruvate methyl ester by immobilized Nocardia opaca under high hydrogen pressure

Noriyuki Nakamura, Anny Sulaswatty, Shigeo Nishimura, Tadashi Matsunaga, Takanori Kitamura, Masao Tsuji, Toshio Kawaguchi

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Phenylalanine methyl ester was produced by immobilized cells of Nocardia opaca from phenylpyruvate methyl ester and NH4Cl under high hydrogen pressure in a biphasic water-ethyl acetate mixture. The production rate (per dry cells) of phenylalanine methyl ester was 0.4 μmol g-1 min-1 under 100 bar hydrogen. When immobilized N. opaca were activated in the medium, the phenylalanine methyl ester production rate per gel increased from 12 to 20 nmol cm-3 min-1. Repeated batch production of phenylalanine methyl ester was carried out with periodic reactivation of immobilized cells. Immobilized cells retained about 70% of the initial activity after nine runs when they were activated in the medium and reacted in ethyl acetate-Tris-HCl buffer (80:20) system.

Original languageEnglish
Pages (from-to)163-171
Number of pages9
JournalJournal of Biotechnology
Issue number2-3
Publication statusPublished - 1992
Externally publishedYes



  • High hydrogen pressure
  • Immobilized cells
  • Nocardia opaca
  • Phenylalanine methyl ester

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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