Phenylalanine methyl ester production from phenylpyruvate methyl ester by immobilized Nocardia opaca under high hydrogen pressure

Noriyuki Nakamura, Anny Sulaswatty, Shigeo Nishimura, Tadashi Matsunaga, Takanori Kitamura, Masao Tsuji, Toshio Kawaguchi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Phenylalanine methyl ester was produced by immobilized cells of Nocardia opaca from phenylpyruvate methyl ester and NH4Cl under high hydrogen pressure in a biphasic water-ethyl acetate mixture. The production rate (per dry cells) of phenylalanine methyl ester was 0.4 μmol g-1 min-1 under 100 bar hydrogen. When immobilized N. opaca were activated in the medium, the phenylalanine methyl ester production rate per gel increased from 12 to 20 nmol cm-3 min-1. Repeated batch production of phenylalanine methyl ester was carried out with periodic reactivation of immobilized cells. Immobilized cells retained about 70% of the initial activity after nine runs when they were activated in the medium and reacted in ethyl acetate-Tris-HCl buffer (80:20) system.

Original languageEnglish
Pages (from-to)163-171
Number of pages9
JournalJournal of Biotechnology
Volume26
Issue number2-3
DOIs
Publication statusPublished - 1992
Externally publishedYes

Keywords

  • High hydrogen pressure
  • Immobilized cells
  • Nocardia opaca
  • Phenylalanine methyl ester

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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