Phospholipase Cδ4 is required for Ca2+ mobilization essential for acrosome reaction in sperm

Kiyoko Fukami, Manabu Yoshida, Takafumi Inoue, Manabu Kurokawa, Rafael A. Fissore, Nobuaki Yoshida, Katsuhiko Mikoshiba, Tadaomi Takenawa

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

Zona pellucida (ZP)-induced acrosome reaction in sperm is a required step for mammalian fertilization. However, the precise mechanism of the acrosome reaction remains unclear. We previously reported that PLCδ4 is involved in the ZP-induced acrosome reaction in mouse sperm. Here we have monitored Ca2+, responses in single sperm, and we report that the [Ca2+]i increase in response to ZP, which is essential for driving the acrosome reaction in vivo, is absent in PLCδ4-/- sperm. Progesterone, another physiological inducer of the acrosome reaction, failed to induce sustained [Ca2+]i increases in PLCδ4-/- sperm, and consequently the acrosome reaction was partially inhibited. In addition, we observed oscillatory [Ca2+]i increases in wild-type sperm in response to these acrosome inducers. Calcium imaging studies revealed that the [Ca2+]i increases induced by exposure to ZP and progesterone started at different sites within the sperm head, indicating that these agonists induce the acrosome reaction via different Ca2+ mechanisms. Furthermore, store-operated channel (SOC) activity was severely impaired in PLCδ4-/- sperm. These results indicate that PLCδ4 is an important enzyme for intracellular [Ca2+]i mobilization in the ZP-induced acrosome reaction and for sustained [Ca2+]i increases through SOC induced by ZP and progesterone in sperm.

Original languageEnglish
Pages (from-to)79-88
Number of pages10
JournalJournal of Cell Biology
Volume161
Issue number1
DOIs
Publication statusPublished - 2003 Apr 14
Externally publishedYes

Fingerprint

Acrosome Reaction
Phospholipases
Zona Pellucida
Spermatozoa
Progesterone
Sperm Head
Acrosome
Fertilization
Calcium
Enzymes

Keywords

  • Acrosome reaction
  • Calcium
  • Phospholipase C
  • Sperm
  • Zona pellucida

ASJC Scopus subject areas

  • Cell Biology

Cite this

Fukami, K., Yoshida, M., Inoue, T., Kurokawa, M., Fissore, R. A., Yoshida, N., ... Takenawa, T. (2003). Phospholipase Cδ4 is required for Ca2+ mobilization essential for acrosome reaction in sperm. Journal of Cell Biology, 161(1), 79-88. https://doi.org/10.1083/jcb.200210057

Phospholipase Cδ4 is required for Ca2+ mobilization essential for acrosome reaction in sperm. / Fukami, Kiyoko; Yoshida, Manabu; Inoue, Takafumi; Kurokawa, Manabu; Fissore, Rafael A.; Yoshida, Nobuaki; Mikoshiba, Katsuhiko; Takenawa, Tadaomi.

In: Journal of Cell Biology, Vol. 161, No. 1, 14.04.2003, p. 79-88.

Research output: Contribution to journalArticle

Fukami, K, Yoshida, M, Inoue, T, Kurokawa, M, Fissore, RA, Yoshida, N, Mikoshiba, K & Takenawa, T 2003, 'Phospholipase Cδ4 is required for Ca2+ mobilization essential for acrosome reaction in sperm', Journal of Cell Biology, vol. 161, no. 1, pp. 79-88. https://doi.org/10.1083/jcb.200210057
Fukami, Kiyoko ; Yoshida, Manabu ; Inoue, Takafumi ; Kurokawa, Manabu ; Fissore, Rafael A. ; Yoshida, Nobuaki ; Mikoshiba, Katsuhiko ; Takenawa, Tadaomi. / Phospholipase Cδ4 is required for Ca2+ mobilization essential for acrosome reaction in sperm. In: Journal of Cell Biology. 2003 ; Vol. 161, No. 1. pp. 79-88.
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