Phospholipase D is essential for keratocyte-like migration of NBT-II cells

Akira Nagasaki, Kimiko Inotsume, Masamitsu Kanada, Taro Q.P. Uyeda

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

NBT-II cells on collagen-coated substrates move rapidly and persistently, maintaining a semi-circular shape with a large lamellipodium, in a manner similar to fish keratocytes. The inhibitor of phospholipase D (PLD), n-butanol, completely blocked the migration and disturbed the characteristic localization of actin along the edge of lamellipodia. To investigate the functional difference between the two isozymes of PLD (PLD1 and PLD2), we transfected NBT-II cells with vectors expressing shRNA to deplete PLD1 or PLD2. Depletion of both PLD1 and 2 by RNA interference reduced the velocity of the migration, but depletion of PLD2 inhibited motility more severely than that of PLD1. Furthermore, GFP-PLD2 was localized to the protruding regions of lamellipodia in migrating cells. Thus, PLD is essential for the maintenance of keratocyte-like locomotion of NBT-II cells, presumably by regulating the actin cytoskeleton.

Original languageEnglish
Pages (from-to)27-33
Number of pages7
JournalCell Structure and Function
Volume33
Issue number1
DOIs
Publication statusPublished - 2008
Externally publishedYes

Keywords

  • Actin
  • Cell migration
  • Knock down
  • NBT-II cell
  • PLD

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

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