Phospholipid-derived fatty acids and quinones as markers for bacterial biomass and community structure in marine sediments

Tadao Kunihiro, Bart Veuger, Diana Vasquez-Cardenas, Lara Pozzato, Marie Le Guitton, Kazuyoshi Moriya, Michinobu Kuwae, Koji Omori, Henricus T S Boschker, Dick Van Oevelen

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Phospholipid-derived fatty acids (PLFA) and respiratory quinones (RQ) are microbial compounds that have been utilized as biomarkers to quantify bacterial biomass and to characterize microbial community structure in sediments, waters, and soils. While PLFAs have been widely used as quantitative bacterial biomarkers in marine sediments, applications of quinone analysis in marine sediments are very limited. In this study, we investigated the relation between both groups of bacterial biomarkers in a broad range of marine sediments from the intertidal zone to the deep sea. We found a good log-log correlation between concentrations of bacterial PLFA and RQ over several orders of magnitude. This relationship is probably due to metabolic variation in quinone concentrations in bacterial cells in different environments, whereas PLFA concentrations are relatively stable under different conditions. We also found a good agreement in the community structure classifications based on the bacterial PLFAs and RQs. These results strengthen the application of both compounds as quantitative bacterial biomarkers. Moreover, the bacterial PLFA- and RQ profiles revealed a comparable dissimilarity pattern of the sampled sediments, but with a higher level of dissimilarity for the RQs. This means that the quinone method has a higher resolution for resolving differences in bacterial community composition. Combining PLFA and quinone analysis as a complementary method is a good strategy to yield higher resolving power in bacterial community structure.

Original languageEnglish
Article numbere96219
JournalPLoS One
Volume9
Issue number4
DOIs
Publication statusPublished - 2014 Apr 25
Externally publishedYes

Fingerprint

Geologic Sediments
Quinones
marine sediments
quinones
Biomass
Biomarkers
Phospholipids
Sediments
community structure
Fatty Acids
biomass
biomarkers
bacterial communities
Bacterial Structures
Optical resolving power
Oceans and Seas
sediments
littoral zone
Soil
phospholipid fatty acids

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Kunihiro, T., Veuger, B., Vasquez-Cardenas, D., Pozzato, L., Le Guitton, M., Moriya, K., ... Van Oevelen, D. (2014). Phospholipid-derived fatty acids and quinones as markers for bacterial biomass and community structure in marine sediments. PLoS One, 9(4), [e96219]. https://doi.org/10.1371/journal.pone.0096219

Phospholipid-derived fatty acids and quinones as markers for bacterial biomass and community structure in marine sediments. / Kunihiro, Tadao; Veuger, Bart; Vasquez-Cardenas, Diana; Pozzato, Lara; Le Guitton, Marie; Moriya, Kazuyoshi; Kuwae, Michinobu; Omori, Koji; Boschker, Henricus T S; Van Oevelen, Dick.

In: PLoS One, Vol. 9, No. 4, e96219, 25.04.2014.

Research output: Contribution to journalArticle

Kunihiro, T, Veuger, B, Vasquez-Cardenas, D, Pozzato, L, Le Guitton, M, Moriya, K, Kuwae, M, Omori, K, Boschker, HTS & Van Oevelen, D 2014, 'Phospholipid-derived fatty acids and quinones as markers for bacterial biomass and community structure in marine sediments', PLoS One, vol. 9, no. 4, e96219. https://doi.org/10.1371/journal.pone.0096219
Kunihiro, Tadao ; Veuger, Bart ; Vasquez-Cardenas, Diana ; Pozzato, Lara ; Le Guitton, Marie ; Moriya, Kazuyoshi ; Kuwae, Michinobu ; Omori, Koji ; Boschker, Henricus T S ; Van Oevelen, Dick. / Phospholipid-derived fatty acids and quinones as markers for bacterial biomass and community structure in marine sediments. In: PLoS One. 2014 ; Vol. 9, No. 4.
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