Phosphorylation by Aurora B converts MgcRacGAP to a RhoGAP during cytokinesis

Yukinori Minoshima, Toshiyuki Kawashima, Koichi Hirose, Yukio Tonozuka, Aie Kawajiri, Ying Chun Bao, Xingming Deng, Masaaki Tatsuka, Shuh Narumiya, W. Stratford May, Tetsuya Nosaka, Kentaro Senba, Takafumi Inoue, Takaya Satoh, Masaki Inagaki, Toshio Kitamura

Research output: Contribution to journalArticle

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Abstract

Cell division is finely controlled by various molecules including small G proteins and kinases/phosphatases. Among these, Aurora B, RhoA, and the GAP MgcRacGAP have been implicated in cytokinesis, but their underlying mechanisms of action have remained unclear. Here, we show that MgcRacGAP colocalizes with Aurora B and RhoA, but not Rac1/Cdc42, at the midbody. We also report that Aurora B phosphorylates MgcRacGAP on serine residues and that this modification induces latent GAP activity toward RhoA in vitro. Expression of a kinase-defective mutant of Aurora B disrupts cytokinesis and inhibits phosphorylation of MgcRacGAP at Ser387, but not its localization to the midbody. Overexpression of a phosphorylation-deficient MgcRacGAP-S387A mutant, but not phosphorylation-mimic MgcRacGAP-S387D mutant, arrests cytokinesis at a late stage and induces polyploidy. Together, these findings indicate that during cytokinesis, MgcRacGAP, previously known as a GAP for Rac/Cdc42, is functionally converted to a RhoGAP through phosphorylation by Aurora B.

Original languageEnglish
Pages (from-to)549-560
Number of pages12
JournalDevelopmental Cell
Volume4
Issue number4
DOIs
Publication statusPublished - 2003 Apr 1
Externally publishedYes

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Phosphorylation
Cytokinesis
Cyclic GMP-Dependent Protein Kinases
Polyploidy
Monomeric GTP-Binding Proteins
Phosphoprotein Phosphatases
Phosphoric Monoester Hydrolases
Cell Division
Protein Kinases
Serine
Phosphotransferases
Cells
Molecules

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Minoshima, Y., Kawashima, T., Hirose, K., Tonozuka, Y., Kawajiri, A., Bao, Y. C., ... Kitamura, T. (2003). Phosphorylation by Aurora B converts MgcRacGAP to a RhoGAP during cytokinesis. Developmental Cell, 4(4), 549-560. https://doi.org/10.1016/S1534-5807(03)00089-3

Phosphorylation by Aurora B converts MgcRacGAP to a RhoGAP during cytokinesis. / Minoshima, Yukinori; Kawashima, Toshiyuki; Hirose, Koichi; Tonozuka, Yukio; Kawajiri, Aie; Bao, Ying Chun; Deng, Xingming; Tatsuka, Masaaki; Narumiya, Shuh; May, W. Stratford; Nosaka, Tetsuya; Senba, Kentaro; Inoue, Takafumi; Satoh, Takaya; Inagaki, Masaki; Kitamura, Toshio.

In: Developmental Cell, Vol. 4, No. 4, 01.04.2003, p. 549-560.

Research output: Contribution to journalArticle

Minoshima, Y, Kawashima, T, Hirose, K, Tonozuka, Y, Kawajiri, A, Bao, YC, Deng, X, Tatsuka, M, Narumiya, S, May, WS, Nosaka, T, Senba, K, Inoue, T, Satoh, T, Inagaki, M & Kitamura, T 2003, 'Phosphorylation by Aurora B converts MgcRacGAP to a RhoGAP during cytokinesis', Developmental Cell, vol. 4, no. 4, pp. 549-560. https://doi.org/10.1016/S1534-5807(03)00089-3
Minoshima Y, Kawashima T, Hirose K, Tonozuka Y, Kawajiri A, Bao YC et al. Phosphorylation by Aurora B converts MgcRacGAP to a RhoGAP during cytokinesis. Developmental Cell. 2003 Apr 1;4(4):549-560. https://doi.org/10.1016/S1534-5807(03)00089-3
Minoshima, Yukinori ; Kawashima, Toshiyuki ; Hirose, Koichi ; Tonozuka, Yukio ; Kawajiri, Aie ; Bao, Ying Chun ; Deng, Xingming ; Tatsuka, Masaaki ; Narumiya, Shuh ; May, W. Stratford ; Nosaka, Tetsuya ; Senba, Kentaro ; Inoue, Takafumi ; Satoh, Takaya ; Inagaki, Masaki ; Kitamura, Toshio. / Phosphorylation by Aurora B converts MgcRacGAP to a RhoGAP during cytokinesis. In: Developmental Cell. 2003 ; Vol. 4, No. 4. pp. 549-560.
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