Phosphorylation of CRMP2 by Cdk5 regulates dendritic spine development of cortical neuron in the mouse hippocampus

Xiaohua Jin, Kodai Sasamoto, Jun Nagai, Yuki Yamazaki, Kenta Saito, Yoshio Goshima, Takafumi Inoue, Toshio Ohshima

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    Proper density and morphology of dendritic spines are important for higher brain functions such as learning and memory. However, our knowledge about molecular mechanisms that regulate thedevelopment and maintenance of dendritic spines is limited. We recently reported that cyclin-dependent kinase 5 (Cdk5) is required for the development and maintenance of dendritic spines of cortical neurons in the mouse brain. Previous in vitro studies have suggested the involvement of Cdk5 substrates in the formation of dendritic spines; however, their role in spine development has not been tested in vivo. Here, we demonstrate that Cdk5 phosphorylates collapsin response mediator protein 2 (CRMP2) in the dendritic spines of cultured hippocampal neurons and in vivo in the mouse brain. When we eliminated CRMP2 phosphorylation in CRMP2KI/KI mice, the densities of dendritic spines significantly decreased in hippocampal CA1 pyramidal neurons in the mouse brain. These results indicate that phosphorylation of CRMP2 by Cdk5 is important for dendritic spine development in cortical neurons in the mouse hippocampus.

    Original languageEnglish
    Article number6790743
    JournalNeural Plasticity
    Volume2016
    DOIs
    Publication statusPublished - 2016

    Fingerprint

    Cyclin-Dependent Kinase 5
    Dendritic Spines
    Hippocampus
    Phosphorylation
    Neurons
    Brain
    Maintenance
    Pyramidal Cells
    collapsin response mediator protein-2
    Spine
    Learning

    ASJC Scopus subject areas

    • Neurology
    • Clinical Neurology

    Cite this

    Phosphorylation of CRMP2 by Cdk5 regulates dendritic spine development of cortical neuron in the mouse hippocampus. / Jin, Xiaohua; Sasamoto, Kodai; Nagai, Jun; Yamazaki, Yuki; Saito, Kenta; Goshima, Yoshio; Inoue, Takafumi; Ohshima, Toshio.

    In: Neural Plasticity, Vol. 2016, 6790743, 2016.

    Research output: Contribution to journalArticle

    Jin, Xiaohua ; Sasamoto, Kodai ; Nagai, Jun ; Yamazaki, Yuki ; Saito, Kenta ; Goshima, Yoshio ; Inoue, Takafumi ; Ohshima, Toshio. / Phosphorylation of CRMP2 by Cdk5 regulates dendritic spine development of cortical neuron in the mouse hippocampus. In: Neural Plasticity. 2016 ; Vol. 2016.
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    AU - Sasamoto, Kodai

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    AU - Yamazaki, Yuki

    AU - Saito, Kenta

    AU - Goshima, Yoshio

    AU - Inoue, Takafumi

    AU - Ohshima, Toshio

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