Phosphorylation of CRMP2 (Collapsin Response Mediator Protein 2) is Involved in Proper Dendritic Field Organization

Naoya Yamashita, Toshio Ohshima, Fumio Nakamura, Papachan Kolattukudy, Jérôme Honnorat, Katsuhiko Mikoshiba, Yoshio Goshima

    Research output: Contribution to journalArticle

    60 Citations (Scopus)

    Abstract

    Collapsin response mediator proteins (CRMPs) are intracellular proteins that mediate signals for several extracellular molecules, such as Semaphorin3A and neurotrophins. The phosphorylation of CRMP1 and CRMP2 by Cdk5 at Ser522 is involved in axonal guidance and spine development. Here, we found that the Ser522-phosphorylated CRMP1 and/or CRMP2 are enriched in the dendrites of cultured cortical neurons and P7 cortical section. To determine the physiological role of CRMPs in dendritic development, we generated CRMP2 knock-in mutant mice (crmp2ki/ki) in which the Ser residue at 522 was replaced with Ala. Strikingly, the cortical basal dendrites of double mutant crmp2ki/ki and crmp1_/_ mice exhibited severe abnormal dendritic patterning, which we defined as "curling phenotype." These findings demonstrate that the function of CRMP1 and CRMP2 synergistically control dendritic projection, and the phosphorylation of CRMP2 at Ser522 is essential for proper dendritic field organization in vivo.

    Original languageEnglish
    Pages (from-to)1360-1365
    Number of pages6
    JournalJournal of Neuroscience
    Volume32
    Issue number4
    DOIs
    Publication statusPublished - 2012 Jan 25

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    Phosphorylation
    Semaphorin-3A
    Dendrites
    Proteins
    Nerve Growth Factors
    Spine
    collapsin response mediator protein-2
    Phenotype
    Neurons

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Phosphorylation of CRMP2 (Collapsin Response Mediator Protein 2) is Involved in Proper Dendritic Field Organization. / Yamashita, Naoya; Ohshima, Toshio; Nakamura, Fumio; Kolattukudy, Papachan; Honnorat, Jérôme; Mikoshiba, Katsuhiko; Goshima, Yoshio.

    In: Journal of Neuroscience, Vol. 32, No. 4, 25.01.2012, p. 1360-1365.

    Research output: Contribution to journalArticle

    Yamashita, Naoya ; Ohshima, Toshio ; Nakamura, Fumio ; Kolattukudy, Papachan ; Honnorat, Jérôme ; Mikoshiba, Katsuhiko ; Goshima, Yoshio. / Phosphorylation of CRMP2 (Collapsin Response Mediator Protein 2) is Involved in Proper Dendritic Field Organization. In: Journal of Neuroscience. 2012 ; Vol. 32, No. 4. pp. 1360-1365.
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    AU - Ohshima, Toshio

    AU - Nakamura, Fumio

    AU - Kolattukudy, Papachan

    AU - Honnorat, Jérôme

    AU - Mikoshiba, Katsuhiko

    AU - Goshima, Yoshio

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