Phosphorylation of the centrosomal protein, Cep169, by Cdk1 promotes its dissociation from centrosomes in mitosis

Yusuke Mori, Yoko Inoue, Yuki Taniyama, Sayori Tanaka, Yasuhiko Terada

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Cep169 is a centrosomal protein conserved among vertebrates. In our previous reports, we showed that mammalian Cep169 interacts and collaborates with CDK5RAP2 to regulate microtubule (MT) dynamics and stabilization. Although Cep169 is required for MT regulation, its precise cellular function remains largely elusive. Here we show that Cep169 associates with centrosomes during interphase, but dissociates from these structures from the onset of mitosis, although CDK5RAP2 (Cep215) is continuously located at the centrosomes throughout cell cycle. Interestingly, treatment with purvalanol A, a Cdk1 inhibitor, nearly completely blocked the dissociation of Cep169 from centrosomes during mitosis. In addition, mass spectrometry analyses identified 7 phosphorylated residues of Cep169 corresponding to consensus phosphorylation sequence for Cdk1. These data suggest that the dissociation of Cep169 from centrosomes is controlled by Cdk1/Cyclin B during mitosis, and that Cep169 might regulate MT dynamics of mitotic spindle.

    Original languageEnglish
    Pages (from-to)642-646
    Number of pages5
    JournalBiochemical and Biophysical Research Communications
    Volume468
    Issue number4
    DOIs
    Publication statusPublished - 2015 Dec 25

    Fingerprint

    Centrosome
    Phosphorylation
    Mitosis
    Microtubules
    Cyclin B
    Mass spectrometry
    Proteins
    Stabilization
    Cells
    Spindle Apparatus
    Interphase
    Consensus Sequence
    Vertebrates
    Mass Spectrometry
    Cell Cycle
    6-((3-chloro)anilino)-2-(isopropyl-2-hydroxyethylamino)-9-isopropylpurine

    Keywords

    • Cdk1
    • Centrosome
    • Cep169
    • Mitosis
    • Phosphorylation

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Cell Biology
    • Molecular Biology

    Cite this

    Phosphorylation of the centrosomal protein, Cep169, by Cdk1 promotes its dissociation from centrosomes in mitosis. / Mori, Yusuke; Inoue, Yoko; Taniyama, Yuki; Tanaka, Sayori; Terada, Yasuhiko.

    In: Biochemical and Biophysical Research Communications, Vol. 468, No. 4, 25.12.2015, p. 642-646.

    Research output: Contribution to journalArticle

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    AU - Tanaka, Sayori

    AU - Terada, Yasuhiko

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