Photo-induced in situ crosslinking of polymer brushes with dimethyl maleimide moieties for dynamically stimulating stem cell differentiation

Yoshinori Arisaka, Yuka Nishijima, Shin Ichi Yusa, Naoya Takeda

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    We designed photo-crosslinkable polymer brushes with dimethylmaleimide moieties, in order to demonstrate dynamic stimulation of cell differentiation in mesenchymal stem cells (MSCs). The polymer brushes were synthesized by surface-initiated reversible addition fragmentation chain transfer polymerization using dimethylmaleimide ethyl methacrylate and methyl methacrylate on a chain transfer agent-immobilized glass surface. The polymer brushes were crosslinked by photodimerization of the dimethylmaleimide moieties within polymer chains with stem cells present on the surface. In order to evaluate the effects of in situ photo-induced crosslinking of the polymer brushes on gene expression of stem cells, human bone marrow MSCs were cultured under static and dynamic culture conditions for 7 days. Expression of the osteocalcin (Ocn) gene in MSCs was used as an indicator of osteoblast differentiation under dynamic culture conditions. Structural conversion from non-crosslinked polymer brushes to crosslinked polymer brushes increased the expression of Ocn by 1.4-fold in the presence of adhered cells, compared with non-crosslinked polymer brushes under static culture conditions. These results suggest that MSCs recognized surface conversion from non-crosslinked to crosslinked structures, which resulted in altered differentiation lineages. Therefore, photo-crosslinkable surfaces with dimethyl maleimide moieties are potential novel materials for dynamically stimulating MSC differentiation.

    Original languageEnglish
    Pages (from-to)1331-1340
    Number of pages10
    JournalJournal of Biomaterials Science, Polymer Edition
    Volume27
    Issue number13
    DOIs
    Publication statusPublished - 2016 Sep 1

    Fingerprint

    Brushes
    Stem cells
    Crosslinking
    Cell Differentiation
    Polymers
    Stem Cells
    Mesenchymal Stromal Cells
    Cell culture
    Methacrylates
    Osteocalcin
    Gene Expression
    maleimide
    Osteoblasts
    Gene expression
    Polymerization
    Glass
    Bone
    Genes
    Bone Marrow

    Keywords

    • dimethyl maleimide
    • dynamic differentiation
    • mesenchymal stem cell
    • Photo-induced crosslinking
    • polymer brush

    ASJC Scopus subject areas

    • Biophysics
    • Bioengineering
    • Biomaterials
    • Biomedical Engineering

    Cite this

    Photo-induced in situ crosslinking of polymer brushes with dimethyl maleimide moieties for dynamically stimulating stem cell differentiation. / Arisaka, Yoshinori; Nishijima, Yuka; Yusa, Shin Ichi; Takeda, Naoya.

    In: Journal of Biomaterials Science, Polymer Edition, Vol. 27, No. 13, 01.09.2016, p. 1331-1340.

    Research output: Contribution to journalArticle

    @article{45b0ac79f9a8405697f6a2286f74a26e,
    title = "Photo-induced in situ crosslinking of polymer brushes with dimethyl maleimide moieties for dynamically stimulating stem cell differentiation",
    abstract = "We designed photo-crosslinkable polymer brushes with dimethylmaleimide moieties, in order to demonstrate dynamic stimulation of cell differentiation in mesenchymal stem cells (MSCs). The polymer brushes were synthesized by surface-initiated reversible addition fragmentation chain transfer polymerization using dimethylmaleimide ethyl methacrylate and methyl methacrylate on a chain transfer agent-immobilized glass surface. The polymer brushes were crosslinked by photodimerization of the dimethylmaleimide moieties within polymer chains with stem cells present on the surface. In order to evaluate the effects of in situ photo-induced crosslinking of the polymer brushes on gene expression of stem cells, human bone marrow MSCs were cultured under static and dynamic culture conditions for 7 days. Expression of the osteocalcin (Ocn) gene in MSCs was used as an indicator of osteoblast differentiation under dynamic culture conditions. Structural conversion from non-crosslinked polymer brushes to crosslinked polymer brushes increased the expression of Ocn by 1.4-fold in the presence of adhered cells, compared with non-crosslinked polymer brushes under static culture conditions. These results suggest that MSCs recognized surface conversion from non-crosslinked to crosslinked structures, which resulted in altered differentiation lineages. Therefore, photo-crosslinkable surfaces with dimethyl maleimide moieties are potential novel materials for dynamically stimulating MSC differentiation.",
    keywords = "dimethyl maleimide, dynamic differentiation, mesenchymal stem cell, Photo-induced crosslinking, polymer brush",
    author = "Yoshinori Arisaka and Yuka Nishijima and Yusa, {Shin Ichi} and Naoya Takeda",
    year = "2016",
    month = "9",
    day = "1",
    doi = "10.1080/09205063.2016.1196531",
    language = "English",
    volume = "27",
    pages = "1331--1340",
    journal = "Journal of Biomaterials Science, Polymer Edition",
    issn = "0920-5063",
    publisher = "Taylor and Francis Ltd.",
    number = "13",

    }

    TY - JOUR

    T1 - Photo-induced in situ crosslinking of polymer brushes with dimethyl maleimide moieties for dynamically stimulating stem cell differentiation

    AU - Arisaka, Yoshinori

    AU - Nishijima, Yuka

    AU - Yusa, Shin Ichi

    AU - Takeda, Naoya

    PY - 2016/9/1

    Y1 - 2016/9/1

    N2 - We designed photo-crosslinkable polymer brushes with dimethylmaleimide moieties, in order to demonstrate dynamic stimulation of cell differentiation in mesenchymal stem cells (MSCs). The polymer brushes were synthesized by surface-initiated reversible addition fragmentation chain transfer polymerization using dimethylmaleimide ethyl methacrylate and methyl methacrylate on a chain transfer agent-immobilized glass surface. The polymer brushes were crosslinked by photodimerization of the dimethylmaleimide moieties within polymer chains with stem cells present on the surface. In order to evaluate the effects of in situ photo-induced crosslinking of the polymer brushes on gene expression of stem cells, human bone marrow MSCs were cultured under static and dynamic culture conditions for 7 days. Expression of the osteocalcin (Ocn) gene in MSCs was used as an indicator of osteoblast differentiation under dynamic culture conditions. Structural conversion from non-crosslinked polymer brushes to crosslinked polymer brushes increased the expression of Ocn by 1.4-fold in the presence of adhered cells, compared with non-crosslinked polymer brushes under static culture conditions. These results suggest that MSCs recognized surface conversion from non-crosslinked to crosslinked structures, which resulted in altered differentiation lineages. Therefore, photo-crosslinkable surfaces with dimethyl maleimide moieties are potential novel materials for dynamically stimulating MSC differentiation.

    AB - We designed photo-crosslinkable polymer brushes with dimethylmaleimide moieties, in order to demonstrate dynamic stimulation of cell differentiation in mesenchymal stem cells (MSCs). The polymer brushes were synthesized by surface-initiated reversible addition fragmentation chain transfer polymerization using dimethylmaleimide ethyl methacrylate and methyl methacrylate on a chain transfer agent-immobilized glass surface. The polymer brushes were crosslinked by photodimerization of the dimethylmaleimide moieties within polymer chains with stem cells present on the surface. In order to evaluate the effects of in situ photo-induced crosslinking of the polymer brushes on gene expression of stem cells, human bone marrow MSCs were cultured under static and dynamic culture conditions for 7 days. Expression of the osteocalcin (Ocn) gene in MSCs was used as an indicator of osteoblast differentiation under dynamic culture conditions. Structural conversion from non-crosslinked polymer brushes to crosslinked polymer brushes increased the expression of Ocn by 1.4-fold in the presence of adhered cells, compared with non-crosslinked polymer brushes under static culture conditions. These results suggest that MSCs recognized surface conversion from non-crosslinked to crosslinked structures, which resulted in altered differentiation lineages. Therefore, photo-crosslinkable surfaces with dimethyl maleimide moieties are potential novel materials for dynamically stimulating MSC differentiation.

    KW - dimethyl maleimide

    KW - dynamic differentiation

    KW - mesenchymal stem cell

    KW - Photo-induced crosslinking

    KW - polymer brush

    UR - http://www.scopus.com/inward/record.url?scp=84976277545&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84976277545&partnerID=8YFLogxK

    U2 - 10.1080/09205063.2016.1196531

    DO - 10.1080/09205063.2016.1196531

    M3 - Article

    C2 - 27255343

    AN - SCOPUS:84976277545

    VL - 27

    SP - 1331

    EP - 1340

    JO - Journal of Biomaterials Science, Polymer Edition

    JF - Journal of Biomaterials Science, Polymer Edition

    SN - 0920-5063

    IS - 13

    ER -